Amyloid Proteins

Methods and Protocols

  • Einar M. Sigurdsson
  • Miguel Calero
  • María Gasset

Part of the Methods in Molecular Biology book series (MIMB, volume 849)

Table of contents

  1. Front Matter
    Pages i-xv
  2. In Vitro Models and Assays

    1. Front Matter
      Pages 1-1
    2. Adam P. Gunn, Blaine R. Roberts, Ashley I. Bush
      Pages 3-10
    3. Dahabada H. J. Lopes, Sharmistha Sinha, Clark Rosensweig, Gal Bitan
      Pages 11-21
    4. Clark Rosensweig, Kenjiro Ono, Kazuma Murakami, Devin K. Lowenstein, Gal Bitan, David B. Teplow
      Pages 23-31
    5. Natallia Makarava, Ilia V. Baskakov
      Pages 33-52
    6. Sadasivam Jeganathan, Subashchandrabose Chinnathambi, Eva-Maria Mandelkow, Eckhard Mandelkow
      Pages 85-99
    7. Alexander K. Buell, Christopher M. Dobson, Mark E. Welland
      Pages 101-119
    8. Kyle L. Morris, Louise C. Serpell
      Pages 121-135
    9. Susanne Wegmann, Daniel J. Muller, Eckhard Mandelkow
      Pages 169-183
    10. Marçal Vilar, Lei Wang, Roland Riek
      Pages 185-198
    11. Marcelo A. Barria, Dennisse Gonzalez-Romero, Claudio Soto
      Pages 199-212
    12. Miguel Calero, Agueda Rostagno, Jorge Ghiso
      Pages 213-223
  3. Cell Culture Models and Assays

    1. Front Matter
      Pages 225-225
    2. Ingvar H. Olafsson, Dadi Th. Vilhjalmsson, Finnbogi R. Thormodsson
      Pages 245-259

About this book


Amyloid diseases are characterized by the deposition of insoluble fibrous amyloid proteins. The word “amyloid” indicates a starch-like compound, and though a misnomer, continues to be the accepted term for this group of protein conformational disorders. The second edition  of Amyloid Proteins expands upon the previous edition with current, detailed protocols for the preparation of amyloid and its precursors, specific analytical methods for studying these proteins, cell culture models and assays for production of amyloid proteins, and protocols for amyloid extraction from tissue, its detection in vitro and in vivo, as well as nontransgenic methods for developing amyloid mouse models. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Amyloid Proteins, Second Edition seeks to aid scientists in the amyloid field to establish new techniques in their laboratories.

Authoritative and practical, Amyloid Proteins, Second Edition seeks to aid scientists in the amyloid field to establish new techniques in their laboratories.


Amyloid aggregates cell culture models diseases in vitro in vivo nontransgenic methods pathogenesis protein polymers transgenic technology

Editors and affiliations

  • Einar M. Sigurdsson
    • 1
  • Miguel Calero
    • 2
  • María Gasset
    • 3
  1. 1.School of Medicine, Dept. PsychiatryNew York UniversityNew YorkUSA
  2. 2.Centro Nacional de MicrobiologíaInstituto de Salud Carlos IIIMadridSpain
  3. 3.Inst. Química Física, RocasolanoCSIC MadridMadridSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-550-3
  • Online ISBN 978-1-61779-551-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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