Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 832)
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Table of contents (46 protocols)
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Recognition and Chain Formation of UbF Modifiers
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Proteasome Biogenesis, Regulation, and Function
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Front Matter
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Proteasome Biogenesis, Regulation and Function
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Protein Quality Control
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Front Matter
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About this book
The ubiquitin-proteasome system (UPS) and ubiquitin-related modifiers are not only involved in cellular protein quality control but also in the regulation of many fundamental cellular processes/pathways as well as in their disease-relevant aberrations. Ubiquitin Family Modifiers and Proteasome: Reviews and Protocols presents both novel developments in UPS research and important methods related to the main recent advances in the field of ubiquitin family modifiers. Divided into five convenient sections, this volume focuses on the enzymology and substrate identification of ubiquitin family modifiers, the recognition and chain formation of these modifiers, the analysis of proteasome biogenesis and function, protein quality control, and finally the use of small molecules and strategies to study or manipulate the function of the UPS and of ubiquitin family modifiers, respectively. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Ubiquitin Family Modifiers and Proteasome: Reviews and Protocols will be of great use to investigators and students engaged in both basic and applied research in life sciences.
Editors and Affiliations
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Institut für Genetik, Universität Köln, Köln, Germany
R. Jürgen Dohmen
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Fak. Biologie, Universität Konstanz, Konstanz, Germany
Martin Scheffner
Bibliographic Information
Book Title: Ubiquitin Family Modifiers and the Proteasome
Book Subtitle: Reviews and Protocols
Editors: R. Jürgen Dohmen, Martin Scheffner
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-474-2
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-473-5Published: 14 February 2012
Softcover ISBN: 978-1-4939-5905-1Published: 23 August 2016
eBook ISBN: 978-1-61779-474-2Published: 14 February 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVI, 666
Number of Illustrations: 94 b/w illustrations, 39 illustrations in colour
Topics: Protein Science, Proteomics
Industry Sectors: Biotechnology, Pharma