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  • Book
  • © 2012

mTOR

Methods and Protocols

  • Unites current biochemical assays and in vitro as well as in vivo models
  • Provides step-by-step detail essentail for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 821)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiii
  2. Evaluation of the Nutrient-Sensing mTOR Pathway

    • Sungki Hong, Aristotle M. Mannan, Ken Inoki
    Pages 29-44
  3. mTOR Activity Under Hypoxia

    • Douangsone D. Vadysirisack, Leif W. Ellisen
    Pages 45-58
  4. Isolation of the mTOR Complexes by Affinity Purification

    • Dos D. Sarbassov, Olga Bulgakova, Rakhmet I. Bersimbaev, Tattym Shaiken
    Pages 59-74
  5. Overexpression or Downregulation of mTOR in Mammalian Cells

    • Mahmoud Khalil, Ivan Gout
    Pages 87-103
  6. Detection of Cytoplasmic and Nuclear Functions of mTOR by Fractionation

    • Margit Rosner, Markus Hengstschläger
    Pages 105-124
  7. Evaluation of Rapamycin-Induced Cell Death

    • Lorenzo Galluzzi, Eugenia Morselli, Oliver Kepp, Ilio Vitale, Aména Ben Younes, Maria Chiara Maiuri et al.
    Pages 125-169
  8. Evaluation of mTOR-Regulated mRNA Translation

    • Valentina Iadevaia, Xuemin Wang, Zhong Yao, Leonard J. Foster, Christopher G. Proud
    Pages 171-185
  9. A Genome-wide RNAi Screen for Polypeptides that Alter rpS6 Phosphorylation

    • Angela Papageorgiou, Joseph Avruch
    Pages 187-214
  10. Immunohistochemical Analysis of mTOR Activity in Tissues

    • Jinhee Kim, Nancy Otto, Claudio J. Conti, Irma B. Gimenz-Conti, Cheryl L. Walker
    Pages 215-225
  11. Quantitative Visualization of Autophagy Induction by mTOR Inhibitors

    • Beat Nyfeler, Philip Bergman, Christopher J. Wilson, Leon O. Murphy
    Pages 239-250
  12. Inducible raptor and rictor Knockout Mouse Embryonic Fibroblasts

    • Nadine Cybulski, Vittoria Zinzalla, Michael N. Hall
    Pages 267-278
  13. Expanding Human T Regulatory Cells with the mTOR-Inhibitor Rapamycin

    • Manuela Battaglia, Angela Stabilini, Eleonora Tresoldi
    Pages 279-293
  14. Rapamycin-Induced Enhancement of Vaccine Efficacy in Mice

    • Chinnaswamy Jagannath, Pearl Bakhru
    Pages 295-303

About this book

Since its discovery, the mammalian target of rapamycin (mTOR) has been shown to regulate many critical molecular processes in eukaryotes such as metabolism, growth, survival, aging, synaptic plasticity, memory, and immunity. In mTOR: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study mTOR. These include methods and techniques  used for the study of the mTOR pathway and potential therapeutic applications of mTOR inhibitors  such as, immunosuppressive and anticancer agents. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, mTOR: Methods and Protocols seeks to aid scientists in the further study of this crucially important molecule  ranging from the analysis of signal transduction events within a cell to the assessment of complex human diseases such as metabolic disorders or cancer.

Editors and Affiliations

  • Division of Nephrology and Dialysis, Medical University of Vienna, Vienna, Austria

    Thomas Weichhart

Bibliographic Information

  • Book Title: mTOR

  • Book Subtitle: Methods and Protocols

  • Editors: Thomas Weichhart

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-430-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-429-2Published: 02 December 2011

  • Softcover ISBN: 978-1-4939-6209-9Published: 23 August 2016

  • eBook ISBN: 978-1-61779-430-8Published: 02 February 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 444

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access