Editors:
- Presents detailed protocols developed using mammalian, yeast, and Drosophila cells and tissues, which can easily be applied across species and cell types
- Features reliable methods tested in laboratories around the world
- Includes expert tips and implementation advice to ensure successful results
Part of the book series: Methods in Molecular Biology (MIMB, volume 809)
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Table of contents (39 protocols)
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Front Matter
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Promoter Elements, Transcription Factors, and Pre-initiation Complex Assembly
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Front Matter
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Promoter Elements, Transcription Factors, and Pre-Initiation Complex (PIC) Assembly
About this book
Through many recent remarkable developments, perhaps the most significant advancements in the study of transcriptional regulation are the development of genome-wide approaches for measuring gene expression, exemplified by gene chips (chip), and chromatin immunoprecipitation assays (ChIP) for measuring in vivo protein-DNA interactions at any genomic loci. Transcriptional Regulation: Methods and Protocols takes this progress and builds upon it with a collection of key protocols used in expert laboratories around the world. Divided into four convenient sections, this detailed volume explores promoter elements, transcription factors, and preinitiation complex (PIC) assembly, chromatin structure, chromatin modifying complexes, and RNA synthesis and regulation. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.
Comprehensive and accessible, Transcriptional Regulation: Methods and Protocols equally serves senior researchers and scientists experienced in transcriptional regulation as well as graduate students and scientists who wish to study transcriptional regulation for the first time.
Editors and Affiliations
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, Department of Biological Sciences, St. John's University, Queens, USA
Ales Vancura
Bibliographic Information
Book Title: Transcriptional Regulation
Book Subtitle: Methods and Protocols
Editors: Ales Vancura
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-376-9
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-375-2Published: 24 November 2011
Softcover ISBN: 978-1-4939-5854-2Published: 23 August 2016
eBook ISBN: 978-1-61779-376-9Published: 25 November 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVI, 627
Number of Illustrations: 104 b/w illustrations
Topics: Biochemistry, general, Gene Expression
Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma