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Microtubule Dynamics

Methods and Protocols

  • Anne Straube

Part of the Methods in Molecular Biology book series (MIMB, volume 777)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Anne Straube
    Pages 1-14
  3. Christopher Gell, Claire T. Friel, Barbara Borgonovo, David N. Drechsel, Anthony A. Hyman, Jonathon Howard
    Pages 15-28
  4. Douglas R. Drummond, Susan Kain, Anthony Newcombe, Christina Hoey, Miho Katsuki, Robert A. Cross
    Pages 29-55
  5. Benjamin Lacroix, Carsten Janke
    Pages 57-69
  6. Laurence Lafanechère, Didier Job
    Pages 71-86
  7. Jaime N. Campbell, Kevin C. Slep
    Pages 87-97
  8. Miho Katsuki, Etsuko Muto, Robert A. Cross
    Pages 117-126
  9. Svenja-Marei Kalisch, Liedewij Laan, Marileen Dogterom
    Pages 147-165
  10. Susanne Bechstedt, Michal Wieczorek, Michael Noujaim, Gary J. Brouhard
    Pages 167-176
  11. Claire T. Friel, Clive R. Bagshaw, Jonathon Howard
    Pages 177-192
  12. Frédéric M. Coquelle, Sophie Blestel, Claire Heichette, Isabelle Arnal, Charles Kervrann, Denis Chrétien
    Pages 193-208
  13. Ka Lou Yu, Nanda Keijzer, Casper C. Hoogenraad, Anna Akhmanova
    Pages 293-316
  14. Back Matter
    Pages 317-319

About this book

Introduction

Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back.  In Microtubule Dynamics: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells. Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin from different sources and with different posttranslational modifications, methods used to study microtubule dynamics and microtubule interactions in vitro, techniques to investigate the ultrastructure of microtubules and associated proteins, assays to study microtubule nucleation, turnover, and force production in cells, as well as approaches to isolate novel microtubule-associated proteins and their interacting proteins.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Definitive and practical, Microtubule Dynamics: Methods and Protocols provides the key protocols needed by novices and experts on how to perform a broad range of well-established and newly-emerging techniques in this vital field.

Keywords

Cellular assays Dynamic instability Interacting proteins Microtubule-mediated processes Regulation Sub-cellular structures Tubulin isolation Ultrastructure

Editors and affiliations

  • Anne Straube
    • 1
  1. 1.Warwick Medical School, Ctr for Mechanochemical Cell BiologyUniversity of WarwickCoventryUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-252-6
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-251-9
  • Online ISBN 978-1-61779-252-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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