Transgenic Mouse Methods and Protocols

1. Adenovirus-Mediated Gene Transfer

   • Ko Willems van Dijk, Kyriakos E. Kypreos, Frits J. Fallaux, Jurre Hageman

   Pages 321-343

2. Back Matter

   Pages 345-352

   PDF

The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful. In Transgenic Mouse Methods and Protocols, Second Edition, expert research explore current advances in the field through detailed laboratory protocols. Chapters provide a general introduction outlining how to deal with mice and how to generate transgenic mouse models, explore the generation of conditional and induced knockout and transgenic mice, and offer alternative routes to studying gene function in mice. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and state of the art, Transgenic Mouse Methods and Protocols, second Edition is the ideal guide for all researchers interested in the latest information about the production and analysis of transgenic and knockout mice.

• Gene function
• Genotype-phenotype relationships
• Knockout mice
• Transgenic mice
• Transgenic mouse models
• genetic engineering

From the reviews of the second edition:

“Provided us with the valuable second edition of Transgenic mouse … . A great merit of this book is then the fact that the two Editors decided to devote some chapters to this basic topic! … I strongly suggest to have a copy of this book to those would like to enter the fascinating field of transgenesis; those already active in the field will anyhow surely find interesting aspects to deal with by comparing the techniques they are using with the suggested novelties here presented.” (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)

• Dept. Pathology & Medical Biology, Medical Biology Section, University Medical Center Groningen, Groningen, Netherlands

  Marten H. Hofker

• Mayo Clinic, Rochester, USA

  Jan Deursen

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