PCR Mutation Detection Protocols

  • Bimal D.M. Theophilus
  • Ralph Rapley

Part of the Methods in Molecular Biology book series (MIMB, volume 688)

Table of contents

  1. Front Matter
    Pages i-x
  2. Mike Mitchell, Jacqueline Cutler
    Pages 17-33
  3. Stephen Keeney
    Pages 67-74
  4. Duncan Ayers, Mark Platt, Farzad Javad, Philip J. R. Day
    Pages 75-95
  5. Claude Houdayer, Catherine Dehainault, Marion Gauthier-Villars, Dominique Stoppa-Lyonnet
    Pages 127-142
  6. Jayne A. L. Minton, Sarah E. Flanagan, Sian Ellard
    Pages 143-153
  7. Cathy Meaney, Gail Norbury
    Pages 155-172
  8. Yvonne Wallis, Natalie Morrell
    Pages 173-185
  9. Kathy Mann, Erwin Petek, Barbara Pertl
    Pages 207-226
  10. Joanne Mason, Mike Griffiths
    Pages 269-280
  11. Gillian Mellars, Keith Gomez
    Pages 281-291

About this book

Introduction

Since the publication of the popular first edition, the explosion of DNA sequence information, the access to bioinformatics and mutation databases coupled with the ability to readily detect and confirm mutations has cemented the role of molecular diagnostics in medicine and, in particular, mutation detection by the polymerase chain reaction (PCR). In PCR Mutation Detection Protocols, Second Edition, expert researchers bring the subject up-to-date with key protocols involving the PCR and its many various incarnations such as SSCP, CSGE, and dHPLC. The volume also addresses key areas such as Southern blotting, accurate diagnostics with high throughput, as well as microarray systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which provide the often hard to find information that may mean the difference between the success and failure of the method. Authoritative and cutting-edge, PCR Mutation Detection Protocols, Second Edition aims to stimulate postgraduate scientists, researchers, and clinicians already engaged in the area and to provide an important first step for those new to this practice wanting to adopt the powerful and essential technique in their own laboratories.

Keywords

High throughput techniques Microarray systems Molecular diagnostics Polymerase chain reaction SSCP, CSGE, and dHPLC Southern blotting

Editors and affiliations

  • Bimal D.M. Theophilus
    • 1
  • Ralph Rapley
    • 2
  1. 1.NHS Foundation Trust, Department of HaematologyBirmingham Childrens HospitalBirminghamUnited Kingdom
  2. 2.Dept. BiosciencesUniversity of HertfordshireHatfield, Herts.United Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-947-5
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-946-8
  • Online ISBN 978-1-60761-947-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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