PCR Protocols

  • Daniel J. Park

Part of the Methods in Molecular Biology book series (MIMB, volume 687)

Table of contents

  1. Front Matter
    Pages i-xi
  2. PCR Husbandry

    1. Front Matter
      Pages 1-1
    2. Zaheer Khan
      Pages 3-14
  3. Cloning and Sequencing

    1. Front Matter
      Pages 15-15
    2. Holly H. Hogrefe, Michael C. Borns
      Pages 17-23
    3. Jeannette P. Staheli, Richard Boyce, Dina Kovarik, Timothy M. Rose
      Pages 57-73
  4. Expression, Epigenetic and Copy Number Analysis

    1. Front Matter
      Pages 89-89
    2. Caifu Chen, Ruoying Tan, Linda Wong, Richard Fekete, Jason Halsey
      Pages 113-134
    3. Hideo Matsumura, Kentaro Yoshida, Shujun Luo, Detlev H. Krüger, Günter Kahl, Gary P. Schroth et al.
      Pages 135-146
    4. Junpei Kurosawa, Hiromi Nishiyori, Yoshihide Hayashizaki
      Pages 147-163
    5. James G. Wetmur, Jia Chen
      Pages 165-175
    6. Thomas Ohnesorg, Erin Turbitt, Stefan J. White
      Pages 193-205
    7. Ee Ming Wong, Alexander Dobrovic
      Pages 207-217
  5. Probes and Assays

    1. Front Matter
      Pages 219-219
    2. Maurizio Cardelli
      Pages 221-229

About this book

Introduction

Known for flexibility and robustness, PCR techniques continue to improve through numerous developments, including the identification of thermostable DNA polymerases which exhibit a range of properties to suit given applications. PCR Protocols, Third Edition selects recently developed tools and tricks, contributed by field-leading authors, for the significant value that they add to more generally established methods. Along with the cutting-edge methodologies, this volume describes many core applications, such as PCR cloning and sequencing, expression, copy number or methylation profile analysis, ‘DNA fingerprinting’, diagnostics, protein engineering, interaction screening as well as a chapter highlighting workflow considerations and contamination control, crucial for all PCR methods. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary reagents and materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, PCR Protocols, Third Edition seeks to further elucidate this essential technique while also providing core principles with broad applications for scientists of all backgrounds.

Keywords

DNA polymerases Genome-wide analysis High-throughput sequencing platforms Methylation profile analysis PCR cloning and sequencing Polymerase chain reaction Protein engineering

Editors and affiliations

  • Daniel J. Park
    • 1
  1. 1., Department of PathologyThe University of MelbourneParkvilleAustralia

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-944-4
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-943-7
  • Online ISBN 978-1-60761-944-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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