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Oral Biology

Molecular Techniques and Applications

  • Book
  • © 2010

Overview

Editors:
  1. Gregory J. Seymour

    1. Faculty of Dentistry, Department of Oral Sciences, University of Otago, Dunedin, New Zealand

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  2. Mary P. Cullinan

    1. Faculty of Dentistry, Department of Oral Sciences, University of Otago, Dunedin, New Zealand

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    You can also search for this editor in PubMed   Google Scholar

  3. Nicholas C.K. Heng

    1. Faculty of Dentistry, Sir John Walsh Research Institute, University of Otago, Dunedin, New Zealand

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  • Contains thorough protocols, easily adapted to particular organisms or areas of interest -Includes key chapters with sections on bioinformatics for the analysis and interpretation of data -Delves into saliva studies, microbial inhabitants in the oral cavity, and oral cells and tissues in health and during disease progression, with expert tips on avoiding protocol pitfalls
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 666)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Saliva Studies

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Gene Therapy of Salivary Diseases

      • Bruce J. Baum, Janik Adriaansen, Ana P. Cotrim, Corinne M. Goldsmith, Paola Perez, Senrong Qi et al.
      Pages 3-20

    3. Collection, Storage, and Processing of Saliva Samples for Downstream Molecular Applications

      • Bradley Stephen Henson, David T. Wong
      Pages 21-30

    4. Proteomic Analysis of Saliva: 2D Gel Electrophoresis, LC-MS/MS, and Western Blotting

      • Shen Hu, Jiang Jiang, David T. Wong
      Pages 31-41

    5. Transcriptomic Analyses of Saliva

      • Viswanathan Palanisamy, David T. Wong
      Pages 43-51

  3. Oral Microbiology

    1. Front Matter

      Pages 53-53
      Download chapter PDF

    2. The Oral Microbiota: General Overview, Taxonomy, and Nucleic Acid Techniques

      • José F. Siqueira Jr, Isabela N. Rôças
      Pages 55-69

    3. Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE)

      • José F. Siqueira Jr, Mitsuo Sakamoto, Alexandre S. Rosado
      Pages 71-85

    4. Protocols to Study the Physiology of Oral Biofilms

      • José A. Lemos, Jacqueline Abranches, Hyun Koo, Robert E. Marquis, Robert A. Burne
      Pages 87-102

    5. Adhesion of Yeast and Bacteria to Oral Surfaces

      • Richard D. Cannon, Karl M. Lyons, Kenneth Chong, Ann R. Holmes
      Pages 103-124

    6. Quantitative Analysis of Periodontal Pathogens by ELISA and Real-Time Polymerase Chain Reaction

      • Stephen M. Hamlet
      Pages 125-140

    7. Bacterial Viability Determination in a Dentinal Tubule Infection Model by Confocal Laser Scanning Microscopy

      • Abdul Aziz, Dikesh Parmar, Andrew McNaughton, Geoffrey R. Tompkins
      Pages 141-150

    8. Characterization of Anti-competitor Activities Produced by Oral Bacteria

      • Fengxia Qi, Jens Kreth
      Pages 151-166

    9. Natural Transformation of Oral Streptococci

      • Fernanda Cristina Petersen, Anne Aamdal Scheie
      Pages 167-180

    10. Use of In Vivo-Induced Antigen Technology (IVIAT) to Identify Virulence Factors of Porphyromonas gingivalis

      • Shannon M. Wallet, Jin Chung, Martin Handfield
      Pages 181-195

    11. Oral Bacterial Genome Sequencing Using the High-Throughput Roche Genome Sequencer FLX System

      • Nicholas C.K. Heng, Jo-Ann L. Stanton
      Pages 197-218

    12. Use of a Yeast-Based Membrane Protein Expression Technology to Overexpress Drug Resistance Efflux Pumps

      • Erwin Lamping, Richard D. Cannon
      Pages 219-250

  4. Cells and Tissues

    1. Front Matter

      Pages 251-251
      Download chapter PDF

    2. Explant Culture of Embryonic Craniofacial Tissues: Analyzing Effects of Signaling Molecules on Gene Expression

      • Katja Närhi, Irma Thesleff
      Pages 253-267
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Keywords

About this book

It is generally recognized that the knowledge and research base that underpins dentistry lies in the biological and physical sciences. In this context, the major advances in these sciences over the past two decades have come through the application of molecular bi- ogy and nanotechnology. These advances are currently impacting on the diagnosis and treatment of a wide range of human diseases and it is essential that dental research, education, and practice keep pace with this rapidly advancing ?eld. As pointed out by Ford et al. (1): The de?nition of disease is also changing. Previously, disease was understood to be the presence of symptoms or of a particular phenotype. With increasing knowledge of the genetic basis of many diseases, this de?nition is changing to become the presence of a genotype conferring a pre-disposition to clinical symptoms or phenotype (Ford et al. (1)). This changing de?nition of disease means that today’s undergraduate or graduate student in dentistry (and its related ?elds) must be in a position not only to acquire new knowledge in the future but also to be able to evaluate the information and apply it in a clinically relevant setting. This naturally positions oral biology as an integral part of any dentally related professional’s repertoire of knowledge. There are as many topics in oral biology as there are the number of sites and micro- vironments within the oral cavity.

Reviews

From the reviews:

“This book comprehensively reviews a wide range of techniques and protocols from diverse areas of oral biology, encompassing current research on microbes, host tissues and saliva. The book is obviously aimed at active researchers in the various fields, but because the research topics are so diverse, I would recommend purchase by institutes and departments … .” (Adam Roberts, Microbiology Today, May, 2011)

Editors and Affiliations

  • Faculty of Dentistry, Department of Oral Sciences, University of Otago, Dunedin, New Zealand

    Gregory J. Seymour, Mary P. Cullinan

  • Faculty of Dentistry, Sir John Walsh Research Institute, University of Otago, Dunedin, New Zealand

    Nicholas C.K. Heng

Bibliographic Information

  • Book Title: Oral Biology

  • Book Subtitle: Molecular Techniques and Applications

  • Editors: Gregory J. Seymour, Mary P. Cullinan, Nicholas C.K. Heng

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-820-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2010

  • Hardcover ISBN: 978-1-60761-819-5Published: 31 August 2010

  • Softcover ISBN: 978-1-4939-6142-9Published: 23 August 2016

  • eBook ISBN: 978-1-60761-820-1Published: 18 August 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 422

  • Topics: Microbiology, Pathology

  • Industry Sectors: Biotechnology, Chemical Manufacturing, Consumer Packaged Goods, Pharma

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