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LC-MS/MS in Proteomics

Methods and Applications

  • Book
  • © 2010

Overview

Editors:
  1. Pedro R. Cutillas

    1. Bart’s and the London School of Medicine, Institute of Cancer, Queen Mary, Universtiy of London, London, United Kingdom

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  2. John F. Timms

    1. , EGA Institute for Women's Health, University College London, London, United Kingdom

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    You can also search for this editor in PubMed   Google Scholar

  • Examines current LC-MS technologies, instrumentation, applications, and analysis solutions used in quantitative proteomics
  • Details protocols for isobaric tagging, in vivo stable isotopic labelling, label-free quantification, and multiple reaction monitoring
  • Covers the analysis of protein post-translational modifications, protein complexes, organelles, and kinase activities
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 658)

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Table of contents (21 protocols)

  1. Analysis of Protein Complexes and Organelles

    1. Mapping Protein–Protein Interactions by Quantitative Proteomics

      • Joern Dengjel, Irina Kratchmarova, Blagoy Blagoev
      Pages 267-278

  2. Analysis of Biological Fluids and Clinical Samples

    1. Front Matter

      Pages 279-279
      Download chapter PDF

    2. Analysis of Serum Proteins by LC-MS/MS

      • Sarah Tonack, John P. Neoptolemos, Eithne Costello
      Pages 281-291

    3. Urinary Proteome Profiling Using 2D-DIGE and LC-MS/MS

      • Mark E. Weeks
      Pages 293-309

    4. Analysis of Peptides in Biological Fluids by LC-MS/MS

      • Pedro R. Cutillas
      Pages 311-321

  3. Novel Applications of LC-MS

    1. Front Matter

      Pages 323-323
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    2. Quantification of Protein Kinase Activities by LC-MS

      • Maria P. Alcolea, Pedro R. Cutillas
      Pages 325-337

    3. A Protocol for Top-Down Proteomics Using HPLC and ETD/PTR-MS

      • Sarah R. Hart
      Pages 339-353

  4. Back Matter

    Pages 355-357
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Keywords

About this book

With the development of new quantitative strategies and powerful bioinformatics tools to cope with the analysis of the large amounts of data generated in proteomics experiments, liquid chromatography with tandem mass spectrometry (LC-MS/MS) is making possible the analysis of proteins on a global scale, meaning that proteomics can now start competing with cDNA microarrays for the analysis of whole genomes. In LC-MS/MS in Proteomics: Methods and Applications, experts in the field provide protocols and up-to-date reviews of the applications of LC-MS/MS, with a particular focus on MS-based methods of protein and peptide quantification and the analysis of post-translational modifications. Beginning with overviews of the use of LC-M/MS in protein analysis, the book continues with topics such as protocols for the analysis of post-translational modifications, with particular focus on phosphorylation and glycosylation, popular techniques for quantitative proteomics, such as multiple reaction monitoring, metabolic labelling, and chemical tagging, biomarker discovery in biological fluids, as well as novel applications of LC-MS/MS. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, LC-MS/MS in Proteomics: Methods and Applications presents the techniques and concepts necessary in order to aid proteomic practitioners in the application of LC-MS/MS to essentially any biological problem.

Editors and Affiliations

  • Bart’s and the London School of Medicine, Institute of Cancer, Queen Mary, Universtiy of London, London, United Kingdom

    Pedro R. Cutillas

  • , EGA Institute for Women's Health, University College London, London, United Kingdom

    John F. Timms

Bibliographic Information

  • Book Title: LC-MS/MS in Proteomics

  • Book Subtitle: Methods and Applications

  • Editors: Pedro R. Cutillas, John F. Timms

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-780-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2010

  • Hardcover ISBN: 978-1-60761-779-2Published: 12 August 2010

  • Softcover ISBN: 978-1-4939-5744-6Published: 23 August 2016

  • eBook ISBN: 978-1-60761-780-8Published: 14 September 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 357

  • Topics: Biochemistry, general, Protein Science, Proteomics, Chromatography

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

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