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Cancer Susceptibility

Methods and Protocols

  • Book
  • © 2010

Overview

Editors:
  1. Michelle Webb

    1. , School of Clinical Laboratory Sciences, University of Manchester, Manchester, United Kingdom

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  • Contains convenient protocols dealing with the characterization, identification, and screening of cancer genes
  • Lists expert tips to aid the processes
  • Provides step-by-step, readily reproducible instruction
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 653)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Identifying Cancer Susceptibility Genes

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. The Identification of Colon Cancer Susceptibility Genes by Using Genome-Wide Scans

      • Denise Daley
      Pages 3-21

    3. Prioritizing Candidate Genetic Modifiers of BRCA1 and BRCA2 Using a Combinatorial Analysis of Global Expression and Polymorphism Association Studies of Breast Cancer

      • Logan C. Walker, Amanda B. Spurdle
      Pages 23-34

    4. Microarray-Based Comparative Genomic Hybridization (Array-CGH) as a Useful Tool for Identifying Genes Involved in Glioblastoma (GB)

      • Yolanda Ruano, Manuela Mollejo, Angel Rodríguez de Lope, José Luis Hernández-Moneo, Pedro Martínez, Bárbara Meléndez
      Pages 35-45

    5. Multiplex Amplifiable Probe Hybridization (MAPH) Methodology as an Alternative to Comparative Genomic Hybridization (CGH)

      • Ludmila Kousoulidou, Carolina Sismani, Philippos C. Patsalis
      Pages 47-71

    6. Utilizing Saccharomyces Cerevisiae to Identify Aneuploidy and Cancer Susceptibility Genes

      • Erin D. Strome, Sharon E. Plon
      Pages 73-85

    7. Computational Identification of Cancer Susceptibility Loci

      • Marko Laakso, Sirkku Karinen, Rainer Lehtonen, Sampsa Hautaniemi
      Pages 87-103

    8. Digital Candidate Gene Approach (DigiCGA) for Identification of Cancer Genes

      • Meng-Jin Zhu, Xiang Li, Shu-Hong Zhao
      Pages 105-129

  3. Screening Cancer Susceptibility Genes

    1. Front Matter

      Pages 131-131
      Download chapter PDF

    2. The Use of Denaturing High Performance Liquid Chromatography (DHPLC) for Mutation Scanning of Hereditary Cancer Genes

      • Deborah J. Marsh, Viive M. Howell
      Pages 133-145

    3. Enhanced Mismatch Mutation Analysis: Simultaneous Detection of Point Mutations and Large Scale Rearrangements by Capillary Electrophoresis, Application to BRCA1 and BRCA2

      • Claude Houdayer, Virginie Moncoutier, Jérôme Champ, Jérémie Weber, Jean-Louis Viovy, Dominique Stoppa-Lyonnet
      Pages 147-180

    4. Economical Protocol for Combined Single-Strand Conformation Polymorphism and Heteroduplex Analysis on a Standard Capillary Electrophoresis Apparatus

      • Piotr Kozlowski, Wlodzimierz J. Krzyzosiak
      Pages 181-192

    5. Mutational Screening of hMLH1 and hMSH2 that Confer Inherited Colorectal Cancer Susceptibility Using Denature Gradient Gel Electrophoresis (DGGE)

      • Tao Liu
      Pages 193-205

    6. s-RT-MELT: A Novel Technology for Mutation Screening

      • Jin Li, G. Mike Makrigiorgos
      Pages 207-219

    7. Zoom-In Array Comparative Genomic Hybridization (aCGH) to Detect Germline Rearrangements in Cancer Susceptibility Genes

      • Johan Staaf, Åke Borg
      Pages 221-235

    8. Development of a Scoring System to Screen for BRCA1/2 Mutations

      • Gareth R. Evans, Fiona Lalloo
      Pages 237-247

    9. Use of Splicing Reporter Minigene Assay to Evaluate the Effect on Splicing of Unclassified Genetic Variants

      • Pascaline Gaildrat, Audrey Killian, Alexandra Martins, Isabelle Tournier, Thierry Frébourg, Mario Tosi
      Pages 249-257

    10. Functional Analysis of Human BRCA2 Variants Using a Mouse Embryonic Stem Cell-Based Assay

      • Sergey G. Kuznetsov, Suhwan Chang, Shyam K. Sharan
      Pages 259-280

    11. Developing Functional Assays for BRCA1 Unclassified Variants

      • Michelle Webb
      Pages 281-291
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Keywords

About this book

Over the past two decades, spectacular advances have been made in our understanding of the molecular genetics of cancer, leading to the pursuit of identifying genes that, when mutated, result in an increased susceptibility to the disease. In Cancer Susceptibility: Methods and Protocols, experts in the field bring together the most recent technological developments for identifying and screening cancer susceptibility genes. Divided into two clear sections, the book begins with gene identification, which updates and informs scientists working at identifying novel cancer susceptibility genes, while the second part deals with mutation screening technologies that aid scientists and clinicians working to translate this knowledge into the clinic. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cancer Susceptibility: Methods and Protocols is a timely collection that seeks to provide researchers with the tools to predict and combat this terrible disease.

Reviews

From the reviews:

“This well-written and concise book is very timely, given the significant advances in the understanding of molecular genetics of cancer over the past two decades that led to the identification of genes which, when mutated, result in increased susceptibility to disease. … It is written for scientists and clinicians … . This book will be valuable resource for students, scientists, clinical oncologists, and other researchers interested in learning about cancer susceptibility.” (Omer Iqbal, Doody’s Review Service, February, 2011)

“Michelle Webb is attempting to give the readers the tools to achieve just that in the oncology field. The book provides up to date protocols and guides aimed at identification of mutations in the known cancer susceptibility genes as well as guides on how to search new ones. … this book provides valuable and relevant information about susceptibility genes analysis and I’m sure it won’t spend too much time just lying on the shelf in any research or clinical lab in this field.” (J. Markus, Neoplasma, February, 2011)

“Cancer susceptibility methods and protocols is an up to date book illustrating a collection of molecular protocols intended to represent the basic and advanced molecular techniques which used to identify and screen for cancer susceptibility genes. … This book deserves great praise for the readable presentation of this complex field. … a valuable authoritative and accessible resource for the current molecular methods used to explore and screen for cancer genes. Both cancer biologists and very specialized clinicians … will find this book a good reference.” (Abeer Alsaegh and Diana M. Eccles, Human Genetics, Vol. 131, 2012)

Editors and Affiliations

  • , School of Clinical Laboratory Sciences, University of Manchester, Manchester, United Kingdom

    Michelle Webb

Bibliographic Information

  • Book Title: Cancer Susceptibility

  • Book Subtitle: Methods and Protocols

  • Editors: Michelle Webb

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-759-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2010

  • Hardcover ISBN: 978-1-60761-758-7Published: 02 September 2010

  • Softcover ISBN: 978-1-4939-5760-6Published: 23 August 2016

  • eBook ISBN: 978-1-60761-759-4Published: 19 August 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 295

  • Topics: Oncology, Cancer Research, Human Genetics

  • Industry Sectors: Biotechnology, Health & Hospitals, Pharma

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