Surface Plasmon Resonance

Methods and Protocols

  • Nico J. Mol
  • Marcel J. E. Fischer

Part of the Methods in Molecular Biology book series (MIMB, volume 627)

Table of contents

  1. Front Matter
    Pages i-x
  2. Nico J. de Mol, Marcel J. E. Fischer
    Pages 1-14
  3. Marcel J. E. Fischer
    Pages 55-73
  4. Stephanie Q. Hutsell, Randall J. Kimple, David P. Siderovski, Francis S. Willard, Adam J. Kimple
    Pages 75-90
  5. Adam J. Kimple, Robin E. Muller, David P. Siderovski, Francis S. Willard
    Pages 91-100
  6. Hiroyuki Takeda, Naoki Goshima, Nobuo Nomura
    Pages 131-145
  7. Eric Duverger, Nathalie Lamerant-Fayel, Natacha Frison, Michel Monsigny
    Pages 157-178
  8. Sara Peeters, Tim Stakenborg
    Pages 179-189

About this book

Introduction

While commercial instruments have expanded the usage and the related literature has increased, the quality of surface plasmon resonance (SPR) research has been hindered by a lack of knowledge of the processes that influence the SPR signal.  In Surface Plasmon Resonance: Methods and Protocols, experts in the field present a wide variety of applications involving commercially available SPR instruments. The heart of the SPR technique is to construct a dedicated surface for an assay. Protocols describe such surfaces for many needs, including the study of membrane bound proteins. Beyond the protocols-based chapters, the volume also highlights the backgrounds of vital issues in the use of SPR, including processes occurring within the hydrogel environment of sensors and on lipid membrane surfaces as well as the analysis of kinetic information.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Surface Plasmon Resonance: Methods and Protocols fills a need for well-described, hands-on SPR experimental protocols and promises to inspire the adaptation of these techniques to fit the needs of labs around the world.

Keywords

Binding modes Biomolecular interaction analysis DNA Hydrogels Kinetic analysis Lipid Lipid membranes SPR instruments Sensor surfaces Termination molecular biology proteins

Editors and affiliations

  • Nico J. Mol
    • 1
  • Marcel J. E. Fischer
    • 2
  1. 1.Dept. Pharmaceutical SciencesUtrecht UniversityUtrechtNetherlands
  2. 2.Dept. Pharmaceutical SciencesUtrecht UniversityUtrechtNetherlands

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-670-2
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-669-6
  • Online ISBN 978-1-60761-670-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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