Plant Epigenetics

Methods and Protocols

  • Igor Kovalchuk
  • Franz J. Zemp

Part of the Methods in Molecular Biology™ book series (MIMB, volume 631)

Table of contents

  1. Front Matter
    Pages i-x
  2. Andrea M. Foerster, Ortrun Mittelsten Scheid
    Pages 1-11
  3. Andrea M. Foerster, Jennifer Hetzl, Christoph Müllner, Ortrun Mittelsten Scheid
    Pages 13-22
  4. Palak Kathiria, Igor Kovalchuk
    Pages 41-48
  5. Leonor Ruiz-García, Jose Antonio Cabezas, Nuria de María, María-Teresa Cervera
    Pages 63-74
  6. Martine Boccara, Alexis Sarazin, Bernard Billoud, Agnes Bulski, Louise Chapell, David Baulcombe et al.
    Pages 75-86
  7. Erika Varkonyi-Gasic, Roger P. Hellens
    Pages 109-122
  8. Yuko Tagami, Naoko Inaba, Yuichiro Watanabe
    Pages 123-138
  9. Julia Engelhorn, Franziska Turck
    Pages 185-207
  10. Andrea T. Todd, Enwu Liu, Jonathan E. Page
    Pages 221-236
  11. Alicja Ziemienowicz
    Pages 253-268

About this book

Introduction

The past fifteen years have witnessed major advances in epigenetics, one of the most popular and quickly evolving fields of modern science. In Plant Epigenetics: Methods and Protocols, expert researchers explore the most recent developments, examining in great detail the contribution of epigenetic regulation to cell function in plants. Chapters include a variety of protocols for studying the function of small non-coding RNAs, DNA methylation, and histone modifications in plants, often in different degrees of complexity. This volume describes bioinformatic approaches to the analysis of high-throughput data, such as bisulfite sequencing and Chip-on-chip assays. It features much-desired protocols for plant transgenesis and the analysis of genome stability, with a detailed discussion of their applications to epigenetic studies. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Wide-ranging and innovative, Plant Epigenetics: Methods and Protocols is an invaluable manual designed to help researchers uncover the undiscovered and unexplained phenomena in plant biology.

Keywords

DNA Mutation PCR genes genetics hybridization recombination

Editors and affiliations

  • Igor Kovalchuk
    • 1
  • Franz J. Zemp
    • 2
  1. 1.Dept. Biological SciencesUniversity of LethbridgeLethbridgeCanada
  2. 2.Dept. Biological SciencesUniversity of LethbridgeLethbridgeCanada

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-646-7
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-645-0
  • Online ISBN 978-1-60761-646-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book