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Molecular and Cell Biology Methods for Fungi

  • Amir Sharon

Part of the Methods in Molecular Biology book series (MIMB, volume 638)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Fungal Transformation and Gene Knockout

    1. Front Matter
      Pages 1-1
    2. B. Gillian Turgeon, Bradford Condon, Jinyuan Liu, Ning Zhang
      Pages 3-19
    3. Patrick D. Collopy, Hildur V. Colot, Gyungsoon Park, Carol Ringelberg, Christopher M. Crew, Katherine A. Borkovich et al.
      Pages 33-40
    4. María D. García-Pedrajas, Marina Nadal, Timothy Denny, Lourdes Baeza-Montañez, Zahi Paz, Scott E. Gold
      Pages 55-76
  3. Detection and Quantification of Fungi

    1. Front Matter
      Pages 102-102
    2. Shahar Ish-Shalom, Amnon Lichter
      Pages 103-114
    3. Pascale M. A. Seddas-Dozolme, Christine Arnould, Marie Tollot, Elena Kuznetsova, Vivienne Gianinazzi-Pearson
      Pages 137-152
    4. John Fosu-Nyarko, Michael G. K. Jones, Zhaohui Wang
      Pages 153-163
    5. Simon J. Foster, Brendon J. Monahan
      Pages 175-185
  4. Microscopy and Protein Analysis

  5. Back Matter
    Pages 319-321

About this book

Introduction

With the development of fungal transformation systems and the deciphering of an increasing number of fungal genomes, this diverse clade of heterotrophic eukaryotic organisms has proven to be ideal for molecular work and highly serviceable as model systems to study basic processes with results that are applicable to many organisms, including humans. In Molecular and Cell Biology Methods for Fungi, experts in the field provide an up-to-date set of practical protocols covering a range of frequently used methods used to study molecular and cellular aspects of fungal biology. The included classical protocols such as transformation systems and traditional protein analysis methods, which have been widely used for many years, alongside the most advanced techniques such as genome amplification, whole genome knockout methods, and sophisticated in vivo imaging techniques will prove to be easily adaptable and useful in a wide range of species. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, Molecular and Cell Biology Methods for Fungi seeks to provide cutting-edge techniques in order to aid both experienced fungal research laboratories as well as those that are interested in using fungi as hosts to study their favorite genes.

Keywords

Bur PCR Quantitative PCR Spore Vivo fungal genome fungi gene expression genes hybridization molecular biology protein complexes saccharomyces cerevisiae

Editors and affiliations

  • Amir Sharon
    • 1
  1. 1.Dept. Plant SciencesTel Aviv UniversityTel AvivIsrael

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-611-5
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-610-8
  • Online ISBN 978-1-60761-611-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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