Editors:
- Provides an easily accessible reference volume for topoisomerase protocols written by leading researchers in the field
- Covers a wide range of assays from cell-based methods and molecular analysis of topoisomerases, to biochemical and biophysical studies
- Presents diverse approaches to experimental design, covering model biological systems including yeast and mammalian cells as well as cell-free systems and purified components
- Comprehensive guide detailing topoisomerase research from its enzymology to its function at the cytological level
- Provides tools for the future of the topoisomerase research field
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 582)
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About this book
Due to their importance to cell biology, DNA topology-altering enzymes, collectively DNA topoisomerases and gyrases, have been studied thoroughly from a biochemical perspective, yet the expansion of research at the molecular and cellular levels continues to show a great deal of promise. In DNA Topoisomerases: Methods and Protocols, experts in the field present a compendium of protocols that extends into the new areas of research that have been recently uncovered in order to elucidate the methodology needed to study topoisomerases in the molecular and cellular context. The volume examines subjects such as the analysis of DNA topologies and the interaction of topoisomerases with DNA as tools to measure topoisomerase functions, the binding of topoisomerases to specific sites in the genomes of eukaryotes, including origins of DNA replication, as well as exploration of perturbed topoisomerase function. As a volume in the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and notes on trouble-shooting and avoiding known pitfalls.
Cutting-edge and easy-to-use, DNA Topoisomerases: Methods and Protocols provides key content which reflects an expanded understanding of the functions of these essential enzymes in the hope of inspiring researchers to further important discoveries in this dynamic field.
Editors and Affiliations
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Dept. Genetics, Cell Biology &, University of Minnesota Medical School, Minneapolis, U.S.A.
Duncan J. Clarke
Bibliographic Information
Book Title: DNA Topoisomerases
Book Subtitle: Methods and Protocols
Editors: Duncan J. Clarke
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-340-4
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-60761-339-8Published: 25 September 2009
Softcover ISBN: 978-1-61779-644-9Published: 07 March 2012
eBook ISBN: 978-1-60761-340-4Published: 18 September 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 268
Number of Illustrations: 59 b/w illustrations
Topics: Human Genetics, Biochemistry, general, Enzymology
Industry Sectors: Biotechnology, Pharma