Reverse Chemical Genetics

Now a major approach in the identification of certain gene product functions, reverse chemical genetics allows the identification of target genes to precede phenotypic changes created by specific small molecules. In Reverse Chemical Genetics: Methods and Protocols, expert authors introduce fundamental resources, including databases for reverse chemical genetics, as well as the modification of these cDNA resources by the most recent molecular technologies. The book explores newly established methods in addition to potentially applicable techniques that make use of specific small molecules, plus functional assays utilizing these molecules, while focusing on methods that make feasible the functional discovery and comprehensive knowledge of particular target genes. As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Reverse Chemical Genetics: Methods and Protocols promises to inspire researchers to conduct their own experiments in order to progress this vital field of study.

• 2D electrophoresis
• Escherichia coli
• In situ hybridization
• Microarray
• PCR
• Small molecules
• Surface plasmon resonance
• Target genes
• cDNA libraries
• gene expression
• genes
• genetics
• hybridization
• molecular biology

• Iwai Orthopaedic Medical Hospital, Tokyo, Japan

  Hisashi Koga

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