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  • © 2009

Meiosis

Volume 2, Cytological Methods

Editors:

  • State-of-the-art techniques for analysis of meiotic chromosome dynamics

  • Comprehensive methods for studying meiosis in human and in a large number of model organisms including fungi, plants, invertebrate animals, zebrafish, and mice

  • Chapters on electron microscopy and conventional and immunofluorescent light microscopy

  • Techniques for enriching and culturing mouse meiotic cells

  • Techniques for spreading meiotic chromosomes and immunofluorescent detection of chromosome-associated proteins

  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 558)

Buying options

eBook USD 139.00
Price excludes VAT (USA)
  • ISBN: 978-1-60761-103-5
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 179.99
Price excludes VAT (USA)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xi
  2. Cytological Analysis of Meiotic Chromosomes in Fungi

    1. Front Matter

      Pages 1-1
    2. Analysis of Schizosaccharomyces pombe Meiosis by Nuclear Spreading

      • Josef Loidl, Alexander Lorenz
      Pages 15-36
    3. Real-Time Imaging of Meiotic Chromosomes in Saccharomyces cerevisiae

      • Romain Koszul, Sei Kameoka, Beth M. Weiner
      Pages 81-89
    4. Meiotic Cytogenetics in Coprinus cinereus

      • Miriam E. Zolan, Patricia J. Pukkila
      Pages 115-127
  3. Cytological Analysis of Meiotic Chromosomes in Plants and Small Animals

    1. Front Matter

      Pages 129-129
    2. Cytological Analysis of Arabidopsis thaliana Meiotic Chromosomes

      • Susan J. Armstrong, Eugenio Sanchez-Moran, F. Chris, H. Franklin
      Pages 131-145
    3. Cytological Analysis of Meiosis in Caenorhabditis elegans

      • Carolyn M. Phillips, Kent L. McDonald, Abby F. Dernburg
      Pages 171-195
    4. Cytological Analysis of Meiosis in Fixed Drosophila Ovaries

      • Kim S. McKim, Eric F. Joyce, Janet K. Jang
      Pages 197-216
  4. Cytological and Histological Analysis of Mammalian Germ Cells and Meiotic Chromosomes

    1. Front Matter

      Pages 261-261
    2. Staging of Mouse Seminiferous Tubule Cross-Sections

      • Emad A. Ahmed, Dirk G. de Rooij
      Pages 263-277

About this book

The unique chromosome dynamics of meiosis have fascinated scientists for well over a century, but in recent years there has been a proliferation of new data concerning the pairing, recombination, and segregation of chromosomes. In Meiosis, expert researchers explore recent advances in three main areas: quantitative genetic methods for analysis of homologous recombination and chromosome pairing, development of direct physical assays for DNA intermediates and products of recombination, and cytological methods for characterizing chromosome behaviors and the patterns by which specific proteins associate with meiotic chromosomes. Meiosis: Volume 2, Cytological Methods, details methods for preparing and examining meiotic tissues, cells, and chromosomes by electron microscopy, indirect immunofluorescence, fluorescent in situ hybridization, and live-cell imaging. The methods in this volume are grouped for convenience by general types of organisms: fungi, plants and small animals (mostly invertebrates), and mammals. Composed in the highly successful Methods in Molecular Biology(tm) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Meiosis: Volume 2, Cytological Methods is an essential guidebook, providing up-to-date and critical new protocols for the study of meiotic chromosome dynamics.

Keywords

  • Chromosome dynamics in yeast
  • DNA
  • Drosophila
  • Fluorescent in situ hybridization
  • Indirect immunofluorescence
  • Live-cell imaging
  • Telomere
  • Vertebrate
  • electron microscopy
  • fungi
  • gene expression
  • genes
  • molecular biology
  • proteins
  • saccharomyces cerevisiae

Editors and Affiliations

  • Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, U.S.A.

    Scott Keeney

Bibliographic Information

  • Book Title: Meiosis

  • Book Subtitle: Volume 2, Cytological Methods

  • Editors: Scott Keeney

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-103-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-60761-102-8

  • Softcover ISBN: 978-1-4939-5835-1

  • eBook ISBN: 978-1-60761-103-5

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 456

  • Number of Illustrations: 62 b/w illustrations, 12 illustrations in colour

  • Topics: Cell Biology, Microbiology

  • Industry Sectors: Biotechnology, Health & Hospitals, Pharma

Buying options

eBook USD 139.00
Price excludes VAT (USA)
  • ISBN: 978-1-60761-103-5
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 179.99
Price excludes VAT (USA)