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Cell-Based Assays for High-Throughput Screening

Methods and Protocols

  • Paul A. Clemons
  • Nicola J. Tolliday
  • Bridget K. Wagner

Part of the Methods in Molecular Biology book series (MIMB, volume 486)

Table of contents

  1. Front Matter
    Pages i-x
  2. W. Frank An, Nicola J. Tolliday
    Pages 1-12
  3. Soumaya Zlitni, Jan E. Blanchard, Eric D. Brown
    Pages 13-27
  4. Michael R. Wyler, Deborah H. Smith, Eftihia Cayanis, Udo Többen, Nathalie Aulner, Thomas Mayer
    Pages 29-41
  5. Eyleen J. O’Rourke, Annie L. Conery, Terence I. Moy
    Pages 57-75
  6. April Agee, David Carter
    Pages 77-95
  7. W. Frank An
    Pages 97-107
  8. Andy M. F. Liu, David C. New, Rico K. H. Lo, Yung H. Wong
    Pages 109-123
  9. Gabriela Chiosis, Adam B. Keeton
    Pages 139-149
  10. Bruce S. Edwards, Susan M. Young, Irena Ivnitsky-Steele, Richard D. Ye, Eric R. Prossnitz, Larry A. Sklar
    Pages 151-165
  11. Bridget K. Wagner, Zoltan Arany
    Pages 167-175
  12. Serene Josiah
    Pages 177-192
  13. Anne E. Carpenter
    Pages 193-211
  14. Back Matter
    Pages 213-218

About this book

Introduction

As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful Methods in Molecular Biology™ series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: Methods and Protocols presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches.

Keywords

Cell-based phenotypic screening Data analysis Detection technologies Endoplasmatisches Reticulum HTS Model biological systems PCR genes molecular biology

Editors and affiliations

  • Paul A. Clemons
    • 1
  • Nicola J. Tolliday
    • 2
  • Bridget K. Wagner
    • 3
  1. 1.Broad Institute of Harvard, Chemical Biology ProgramMassachusetts Institute of TechnologyCambridgeU.S.A.
  2. 2.Broad Institute of Harvard, Chemical Biology ProgramMassachusetts Institute of TechnologyCambridgeU.S.A.
  3. 3.Broad Institute of Harvard, Chemical Biology ProgramMassachusetts Institute of TechnologyCambridgeU.S.A.

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60327-545-3
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-544-6
  • Online ISBN 978-1-60327-545-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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