Overview
- Various methods for affinity-purifying cellular or in vitro reconstituted RNPs
- A collection of methods for studying RNA-protein interaction occurred inside a cell
- Various methods for assaying protein footprints on RNA
- Various methods for mapping protein regions involved in RNA interaction
- New methods including quantifying specific RNAs, designing siRNAs, measuring RNPase activity, etc.
- Example methods in many cellular events
Part of the book series: Methods in Molecular Biology (MIMB, volume 488)
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Table of contents (25 protocols)
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Front Matter
Keywords
About this book
Due to the vital biological importance of RNA and proteins functioning together within a cell, a protocol volume describing experimental procedures to study their interactions should find a home in many laboratories. RNA-Protein Interaction Protocols, Second Edition updates, complements, and expands upon the popular first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. The expert contributors explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, RNA-Protein Interaction Protocols, Second Edition is an ideal guide for researchers continuing the study of this all-important biological partnership.
Reviews
From the reviews of the second edition:
"Detailed protocols for analysis of specific examples of RNA protein interactions are described. … Anyone studying RNA protein interactions should seriously consider reading this book, not only as a technical reference, but also as a source of ideas. … It’s a book for the lab bench and the PIs office. Highly recommended." (Laurence Tiley, Microbiology Today, February, 2009)
"The second edition of RNA-protein interaction protocols is part of the Methods in Molecular Biology series and includes detailed methods covering a wide range of approaches to characterise RNA-protein interactions. … This book … is well written, well indexed and well referenced. Each protocol is described in enough detail to be followed by an experienced user. For an RNA-based laboratory this book provides an excellent description of the latest techniques with particular attention given to manipulation of RNPs from in vivo sources." (Matthew Bentham, British Toxicology Society Newsletter, June-August, 2009)
Editors and Affiliations
Bibliographic Information
Book Title: RNA-Protein Interaction Protocols
Editors: Ren-Jang Lin
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-475-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-58829-419-7Published: 25 August 2008
Softcover ISBN: 978-1-61737-555-2Published: 19 November 2010
eBook ISBN: 978-1-60327-475-3Published: 04 November 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XVI, 416
Topics: Biochemistry, general, Cell Biology, Protein Science, Human Genetics
Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma