Protein Secretion

Methods and Protocols

  • Anastassios Economou

Part of the Methods in Molecular Biology book series (MIMB, volume 619)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Andreas Kuhn, Natalie Stiegler, Anne-Kathrin Schubert
    Pages 39-62
  3. Jijun Yuan, Ross E. Dalbey, Andreas Kuhn
    Pages 63-77
  4. Shin-ichiro Narita, Hajime Tokuda
    Pages 117-129
  5. Ilja Kusters, Geert van den Bogaart, Janny de Wit, Viktor Krasnikov, Bert Poolman, Arnold Driessen
    Pages 131-143
  6. Kush Dalal, Franck Duong
    Pages 145-156
  7. Giorgos Gouridis, Spyridoula Karamanou, Marina Koukaki, Anastassios Economou
    Pages 157-172
  8. Susan Schlegel, Mirjam Klepsch, David Wickström, Samuel Wagner, Jan-Willem de Gier
    Pages 257-269
  9. Vladimir A. Likic, Pavel Dolezal, Nermin Celik, Michael Dagley, Trevor Lithgow
    Pages 271-284
  10. Shruti Rastogi, Burkhard Rost
    Pages 285-305
  11. Penelope Strittmatter, Jürgen Soll, Bettina Bölter
    Pages 307-321

About this book

Introduction

Over the past thirty years, many elegant genetic and biochemical approaches have been combined in order to advance the study of protein secretion and the necessary navigation through cell membranes, yet, despite this progress, less than two hundred membrane protein structures are known, nowhere near the complete inventory that the discovered protein export systems suggest. In Protein Secretion: Methods and Protocols, leading experts in the field provide robust, well-established protocols to elucidate the multiplicity of tools that have been developed to study protein sorting, membrane targeting, transmembrane crossing, and secretion across multiple membranes. With examples involving both prokaryotic and eukaryotic organisms, the volume covers subjects ranging from bioinformatics and proteomics to fundamental enzymology and genetics to cell biology, structural analyses, and biophysics. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the key materials and reagents, step-by-step, readily reproducible protocols, and detailed notes on troubleshooting and avoiding known pitfalls.

Comprehensive and dependable, Protein Secretion: Methods and Protocols focuses on well-characterized paradigms so that scientists studying a vast array of subjects from biochemistry and genetics to biotechnology and biopharmaceuticals can benefit and expand upon their vital research.

Keywords

Chloroplast Escherichia coli Lipid Polyacrylamid-Gelelektrophorese Proteomics biochemistry bioinformatics molecular biology protein structure proteins

Editors and affiliations

  • Anastassios Economou
    • 1
  1. 1.Technology - Hellas (FORTH), Inst. Molecular Biology & BiotechnologyFoundation for Research &IraklionGreece

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60327-412-8
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-167-7
  • Online ISBN 978-1-60327-412-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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