Membrane Proteomics

Methods and Protocols

  • Matthew J. Peirce
  • Robin Wait

Part of the Methods in Molecular Biology™ book series (MIMB, volume 528)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. In Silico Methods for Prediction of Membrane Protein Hydrophobicity and Topology

    1. Front Matter
      Pages 1-1
    2. Henry Bigelow, Burkhard Rost
      Pages 3-23
    3. Matthew N. Davies, Darren R. Flower
      Pages 25-36
    4. Edward J. Evans, Lawrence Hene, Mai Vuong, Hussain S.I. Abidi, Simon J. Davis
      Pages 37-56
  3. Extraction and Purification of Membrane Proteins

    1. Front Matter
      Pages 57-57
    2. Plant Membrane Proteins

    3. Prokaryotic Membrane Proteins

    4. Mammalian Membrane Proteins

      1. Fiona M. McCarthy, Amanda M. Cooksey, Shane C. Burgess
        Pages 110-118
      2. Jens Schindler, Hans Gerd Nothwang
        Pages 119-126
      3. R. Jacek Wiśniewski
        Pages 127-134
      4. Cecile A.W. Geuijen, Arjen Q. Bakker, John de Kruif
        Pages 141-158
      5. Georgina S. Butler, Richard A. Dean, Derek Smith, Christopher M. Overall
        Pages 159-176
      6. Nadia Korfali, Elizabeth A.L. Fairley, Selene K. Swanson, Laurence Florens, Eric C. Schirmer
        Pages 201-225
      7. Rommel A. Mathias, Justin W. Lim, Hong Ji, Richard J. Simpson
        Pages 227-242
  4. Separation of Membrane Proteins

    1. Front Matter
      Pages 243-243

About this book

Introduction

With the great cellular and therapeutic importance of plasma membrane proteins, unbiased technologies such as proteomics become even more vital as they have the power to define patterns of membrane protein expression characteristic of distinct states of cellular development, differentiation or disease, and thereby identify novel markers of, or targets for intervention in, disease. In Membrane Proteomics: Methods and Protocols, leading experts in the field compile a laboratory bench resource which provides a comprehensive toolkit of proven methods. The volume delves into various modifications to standard two-dimensional gel electrophoresis protocols as well as liquid chromatographic methods, protocols for the enrichment of diverse classes of plasma membrane proteins, in silico approaches, and cutting-edge techniques for quantitative membrane protein profiling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and easy-to-use, Membrane Proteomics: Methods and Protocols is an ideal guide for investigators wishing to apply state of the art membrane proteomic methodologies in their own research programs.

Keywords

Hydrophobicity In silico Lipid Mammalian membrane proteins Plant membrane proteins Plasma and nuclear membrane proteins Polyacrylamid-Gelelektrophorese Prokaryotic membrane proteins Proteomics Two-dimensional gel electrophoresis (2DE) membrane proteins molecular biology proteins

Editors and affiliations

  • Matthew J. Peirce
    • 1
  • Robin Wait
    • 1
  1. 1.Kennedy Institute of Rheumatology DivisionFaculty of Medicine Imperial College LondonUK

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60327-310-7
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-309-1
  • Online ISBN 978-1-60327-310-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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