Therapeutic Applications of RNAi

1. Front Matter

   Pages i-x

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2. Development and Application of a Dual-Purpose Nanoparticle Platform for Delivery and Imaging of siRNA in Tumors

   • Zdravka Medarova, Mohanraja Kumar, Shu-wing Ng, Anna Moore

   Pages 1-13

3. Targeted Gene Silencing into Solid Tumors with Electrically Mediated siRNA Delivery

   • Muriel Golzio, Laurent Mazzolini, Aurélie Paganin-Gioanni, Justin Teissié

   Pages 15-27

4. Liposomal siRNA for Ovarian Cancer

   • Lingegowda S. Mangala, Hee Dong Han, Gabriel Lopez-Berestein, Anil K. Sood

   Pages 29-42

5. Therapeutic Applications of RNAi for Silencing Virus Replication

   • Ralph A. Tripp, Stephen Mark Tompkins

   Pages 43-61

6. RNAi in the Malaria Vector, Anopheles gambiae

   • Flaminia Catteruccia, Elena A. Levashina

   Pages 63-75

7. RNAi Using a Chitosan/siRNA Nanoparticle System: In Vitro and In Vivo Applications

   • Morten Østergaard Andersen, Kenneth Alan Howard, Jørgen Kjems

   Pages 77-86

8. Lentiviral and Adeno-Associated Vector-Based Therapy for Motor Neuron Disease Through RNAi

   • Chris Towne, Patrick Aebischer

   Pages 87-108

9. Studying Autoimmunity by In Vivo RNA Interference

   • Stephan Kissler

   Pages 109-118

10. Transcriptional Gene Silencing Using Small RNAs

    • Daniel H. Kim, John J. Rossi

    Pages 119-125

11. Alternative Splicing as a Therapeutic Target for Human Diseases

    • Kenneth J. Dery, Veronica Gusti, Shikha Gaur, John E. Shively, Yun Yen, Rajesh K. Gaur

    Pages 127-144

12. Back Matter

    Pages 145-148

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The phenomenon of RNA interference has rapidly moved from groundbreaking scientific discovery to promising therapeutic approach. However, even as RNAi-based drugs enter the clinic, significant challenges remain, particulary in the area of delivery. Therapeutic Applications of RNAi provides detailed protocols in key areas of current focus, including testing of delivery vehicles, identification of appropriate model systems, and evaluation of the effects of RNAi in vivo. Produced by a team of internationally renowned authors, the volume describes the therapeutic applications of RNAi and potential pitfalls in oncology, viral infections and CNS disease, using a variety of delivery methods, including liposomes, peptide-based nanoparticles, polycationic polymers, and viral vehicles. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Therapeutic Applications of RNAi is an ideal guide for scientists attempting to solve the numerous challenges in this field and revolutionize the treatment of disease.

• CNS disease
• Liposomes
• Oncology
• Peptide-based nanoparticles
• RNA
• RNA interference
• Viral infections
• siRNA
• siRNA delivery
• transcription
• biochemical engineering

From the reviews: “This volume in the Methods in Molecular Biology series is a compilation of laboratory protocols organized in 10 chapters dealing with the … task of delivering RNA interference molecules (RNAi) to live organisms to achieve therapeutic effects. … The book is written mainly for scientists, clinical researchers, and all investigators interested in the field of biotherapeutic research.” (Ruben Mestril, Doody’s Review Service, January, 2010)

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