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2D PAGE: Sample Preparation and Fractionation

  • Anton Posch

Part of the Methods in Molecular Biology™ book series (MIMB, volume 425)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Rosalind E. Jenkins, Neil R. Kitteringham, Carrie Greenough, B. Kevin Park
    Pages 15-25
  3. John E. Hale, Valentina Gelfanova, Jin-Sam You, Michael D. Knierman, Robert A. Dean
    Pages 53-66
  4. Baptiste Leroy, Paul Falmagne, Ruddy Wattiez
    Pages 67-75
  5. Begona Casado, Paolo Iadarola, Lewis K Pannell
    Pages 77-87
  6. Daniel Ambort, Daniel Lottaz, Erwin Sterchi
    Pages 113-130
  7. Hermann-Josef Thierse, Stefanie Helm, Patrick Pankert
    Pages 139-147
  8. Ragnar Flengsrud
    Pages 149-152
  9. Etienne H. Meyer, A. Harvey Millar
    Pages 163-169
  10. Sybille E Kubis, Kathryn S Lilley, Paul Jarvis
    Pages 171-186
  11. Elisabeth Jamet, Georges Boudart, Giséle Borderies, Stephane Charmont, Claude Lafitte, Michel Rossignol et al.
    Pages 187-201
  12. Aida Pitarch, César Nombela, Concha Gil
    Pages 217-239
  13. Martha L. Medina, Wilson A. Francisco
    Pages 275-285
  14. Kheir Zuobi-Hasona, L. Jeannine Brady
    Pages 287-293
  15. Jason N. Cole, Steven P. Djordjevic, Mark J. Walker
    Pages 295-311
  16. Wanderley de Souza, José Andrés Morgado-Diaz, Narcisa L. Cunha-e-Silva
    Pages 313-331
  17. Back Matter
    Pages 333-340

About this book

Introduction

The complex nature of proteins often requires a multitude of sample preparation options. In 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics and specifically for two-dimensional electrophoresis (2-DE). Detailed laboratory methods describe the process of sample fractionation used to reduce the complexity of the protein sample and the methods of 2-DE analysis and a broad variety of gel-free mass spectrometry-based technologies to detect previously undetectable proteins. Vital information regarding protein isolation and preparation for reproducible proteome analysis previously overlooked by scientists has been clearly laid out in this pioneering two-volume set. Volume 2 illuminates specific applications of sample preparation tools and fractionation strategies to study a variety of biological systems.

 

Invaluable for students of Biochemistry, Biomedicine, Biology and Genomics and essential for the practicing scientists, 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2 delves into the study of proteins with comprehensive and in depth methods and protocols to provide a greater understanding of this intricate and complicated field.

Keywords

2-DE Arabidopsis thaliana Chloroplast Cytoplasma Electrophoresis Expression Proteomics Polyacrylamid-Gelelektrophorese Proteins Proteome Analysis Proteomics Protozoa biochemistry fungi membrane proteins

Editors and affiliations

  • Anton Posch
    • 1
  1. 1.Bio-Rad Laboratories GmbHGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60327-210-0
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-209-4
  • Online ISBN 978-1-60327-210-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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