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Candida albicans

Methods and Protocols

  • Ronald L. Cihlar
  • Richard A. Calderone

Part of the Methods in Molecular Biology book series (MIMB, volume 499)

Table of contents

  1. Front Matter
    Pages i-x
  2. Immunogical Methods

  3. Virulence and Biofilms

    1. Front Matter
      Pages 35-35
    2. Bastiaan P. Krom, Kevin Buijssen, Henk J. Busscher, Henny C. van der Mei
      Pages 45-54
    3. Bastiaan P. Krom, Jesse B. Cohen, Gail McElhaney-Feser, Henk J. Busscher, Henny C. van der Mei, Ronald L. Cihlar
      Pages 55-62
  4. Virulence Measurements: In vitro, ex vivo, and in vivo

    1. Front Matter
      Pages 63-63
    2. Cornelius J. Clancy, Shaoji Cheng, Minh Hong Nguyen
      Pages 65-76
  5. Strain Typing and Identification

    1. Front Matter
      Pages 95-95
    2. Claude Pujol, David R. Soll
      Pages 117-129
  6. Genomics and Proteomics

    1. Front Matter
      Pages 131-131
    2. Chris Blackwell, Jeremy D. Brown
      Pages 133-148
    3. Joy Sturtevant
      Pages 157-167
    4. Ana M. Ramon, William A. Fonzi
      Pages 169-174
    5. Clarissa J. Nobile, Aaron P. Mitchell
      Pages 175-194
  7. Appendix

    1. Front Matter
      Pages 195-195
    2. Neeraj Chauhan, Michael D. Kruppa
      Pages 197-201
  8. Back Matter
    Pages 203-206

About this book

Introduction

Over the course of the past decade, there have been remarkable advances in the study of human pathogenic fungi. These developments have taken place throughout a wide range of disciplines, and have come as the result of newly available genome sequences of pathogens such as candida albicans and other model fungi. In Candida Albicans: Methods and Protocols, expert researchers explore these exciting new insights, focusing on the study of medically important fungi and Candida spp in particular. Chapters examine critical aspects of molecular methods, providing information on reporter gene assays, transformation, gene expression in vivo, and methods for large-scale gene disruption. At the same time, the work includes in-depth descriptions of disease models of candidiaisis, facts about strain identification, and guidelines on the preparation of samples for proteomic investigations and tandem affinity purification. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting edge, Candida Albicans: Methods and Protocols is an invaluable source of methods for investigators in the exhilarating fields of medical and molecular mycology.

Keywords

DNA Disease models of candidiaisis FISH Gene expression in vivo Proteomics Reporter gene assays Toxin Transformation biofilm gene expression genes molecular biology

Editors and affiliations

  • Ronald L. Cihlar
    • 1
  • Richard A. Calderone
    • 1
  1. 1.School of Medicine Dept. Microbiology & ImmunologyGeorgetown University Medical CenterUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60327-151-6
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-760-0
  • Online ISBN 978-1-60327-151-6
  • Series Print ISSN 1064-3745
  • Buy this book on publisher's site
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