Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols

  • Andreas Marx
  • Oliver Seitz

Part of the Methods in Molecular Biology book series (MIMB, volume 429)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Introduction

    1. Front Matter
      Pages 1-1
  3. Applications

    1. Front Matter
      Pages 21-21
    2. David S. Perlin, Sergey Balashov, Steven Park
      Pages 23-31
    3. Arvind A. Bhagwat, Jitu Patel, Trina Chua, Audrey Chan, Saúl Ruiz Cruz, Gustavo A. González Aguilar
      Pages 33-43
    4. Gunter Schmidtke, Marcus Groettrup
      Pages 45-58
    5. Thomas D. Schmittgen, Eun Joo Lee, Jinmai Jiang
      Pages 89-98
    6. Rachael Carters, Jennifer Ferguson, Rupert Gaut, Paul Ravetto, Nicola Thelwell, David Whitcombe
      Pages 99-115
  4. New Technologies

    1. Front Matter
      Pages 135-135
    2. Adam P. Silverman, Hiroshi Abe, Eric T. Kool
      Pages 161-170
    3. David J. French, David G. McDowell, Paul Debenham, Nittaya Gale, Tom Brown
      Pages 171-185
    4. Elke Socher, Oliver Seitz
      Pages 187-197
    5. Maria Erali, Robert Palais, Carl Wittwer
      Pages 199-206
  5. Non-Nucleic Acid Targets

    1. Front Matter
      Pages 207-207
    2. Jun Li, Zehui Charles Cao, Zhiwen Tang, Kemin Wang, Weihong Tan
      Pages 209-224

About this book

Introduction

Significant DNA sequence data has been made available by several important genome projects. The allocation of functions to a mere string of the four letters in the code of life plays a major if not predominant role of ongoing and future research. Guided by experts in the field, Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols, provides easy-to-follow experiments, commences with an introduction to the basic principles of fluorescence, and shows a diverse set of instructive examples, from probe design to applications in clinical settings, molecular beacon technology, the identification of transgenic mice, along with the potential of realtime PCR. While originally being used for fluorogenic nucleic acid probes with a stem-loop structure, molecular beacon now refers to a whole family of fluorescence-up probes. Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols summarizes all the latest findings and explores powerful techniques designed for geneticist, biotechnologists, molecular and cellular biologists, researchers and developers.

Keywords

Allele DNA Fluorescence Genomics Ligation Microarrays PCR Probes Ribozyme SNP Single Nucleotide Polymorphism diagnostics gene expression genes hybridization

Editors and affiliations

  • Andreas Marx
    • 1
  • Oliver Seitz
    • 2
  1. 1.Department of ChemistryUniversity of KonstanzKonstanzGermany
  2. 2.Institute of ChemistryHumboldt University BerlinBerlinGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60327-040-3
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-700-6
  • Online ISBN 978-1-60327-040-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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