Editors:
- Provides an easily accessible reference volume for methods to investigate the critical steps in apoptotic cell death written by leading researchers in the field
- Details the process of how to induce, measure and differentiate apoptosis in various models
- Covers a wide range of model systems for apoptosis detection from cell-based assays to measurements at the level of organ or organism
- Presents a modular approach to experimental design, covering model biological systems, screening modalities, detection technologies, approaches to data interpretation, and tips for troubleshooting
- Comprehensive guide to methodologies applied to elucidate the molecular mechanisms of apoptosis
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 559)
Buy it now
Buying options
Tax calculation will be finalised at checkout
Other ways to access
This is a preview of subscription content, log in via an institution to check for access.
Table of contents (26 protocols)
-
Analysis of the Function of Major Regulators of Apoptosis/Cell Survival
-
Analysis of Apoptosis in Different Organs
-
Front Matter
-
-
Analysis of Apoptosis in Model Organisms
-
Front Matter
-
-
Back Matter
About this book
The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research. In Apoptosis: Methods and Protocols, Second Edition, expert researchers describe the techniques to best investigate the critical steps involved in the apoptotic process. Presented from several different research perspectives, the volume contains sections covering detection of apoptosis, detection of non-apoptotic cell death, modifications of apoptotic proteins during apoptosis, the analysis of its major regulators, as well as analysis of apoptosis in different organs and in model organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Apoptosis: Methods and Protocols, Second Edition constitutes a key technical reference to the significant methodologies used in the field, and offers beginners and experienced researchers powerful tools to illuminate the phenomena of programmed cell death.
Reviews
From the reviews of the second edition:
“Provides step-by-step protocols for identifying apoptotic cells and analyzing their role in normal tissue development and disease. … The book is written primarily for basic science and clinical researchers (graduate students, postdoctoral fellows, scientists) … . Biomedical researchers working in radiation biology, pharmacology, and molecular pathology also will value this book because of its focus on apoptosis in various organs including the heart and brain. … This beautiful up-to-date synthesis of information about the analysis of apoptosis is filled with practical information.” (Bruce A. Fenderson, Doody’s Review Service, July, 2010)Editors and Affiliations
-
Boston Biomedical Research Institute, Watertown, U.S.A.
Peter Erhardt, Ambrus Toth
Bibliographic Information
Book Title: Apoptosis
Book Subtitle: Methods and Protocols, Second Edition
Editors: Peter Erhardt, Ambrus Toth
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-017-5
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-60327-016-8Published: 15 July 2009
Softcover ISBN: 978-1-61779-489-6Published: 01 December 2011
eBook ISBN: 978-1-60327-017-5Published: 21 July 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XII, 400
Number of Illustrations: 47 b/w illustrations, 8 illustrations in colour
Topics: Cell Biology, Cell Cycle Analysis, Apoptosis
Industry Sectors: Biotechnology, Pharma