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Avidin-Biotin Interactions

Methods and Applications

  • Robert J. McMahon

Part of the Methods In Molecular Biology™ book series (MIMB, volume 418)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Rosaria P. Haugland, Mahesh K. Bhalgat
    Pages 1-12
  3. Rosaria P. Haugland, Wendy W. You
    Pages 13-23
  4. Bao-Shiang Lee, Sangeeth Krishnanchettiar, Syed Salman Lateef, Shalini Gupta
    Pages 51-61
  5. Andreas Ebner, Markus Marek, Karl Kaiser, Gerald Kada, Christoph D. Hahn, Bernd Lackner et al.
    Pages 73-88
  6. Christoph Rösli, Jascha-N. Rybak, Dario Neri, Giuliano Elia
    Pages 89-100
  7. Nicolas Humbert, Peter Schürmann, Andrea Zocchi, Jean-Marc Neuhaus, Thomas R. Ward
    Pages 101-110
  8. Bruce E. McKay, Michael L. Molineux, Ray W. Turner
    Pages 111-128
  9. Gabriela Camporeale, Yap Ching Chew, Alice Kueh, Gautam Sarath, Janos Zempleni
    Pages 139-148
  10. Back Matter
    Pages 221-232

About this book

Introduction

For researchers today, the avidin-biotin interaction is among the most widely exploited among biomedical scientists. Despite its popularity, avidin-biotin methodology suffers from a lack of clarity associated with popular, low cost, prefabricated kits used by many in the field. In Avidin-Biotin Interactions: Methods and Applications, leaders in avidin-biotin methodology share their knowledge relating to the application of the extremely powerful interaction between the protein avidin or its homologues and the vitamin biotin and some of its homologues. Although avidin-biotin based kits based have been produced for quite some time and remain a widely used methodology, many of the chapters in this text describe situations in which the previous methodology failed due to some factor, and the authors developed new and better ways for the avidin-biotin system to be utilized. With excellent descriptions of laboratory protocols written by expert researchers, this volume is equally perfect for the student or the professional laboratory scientist. Each chapter in Avidin-Biotin Interactions: Methods and Applications contains not only excellent descriptions of protocols for the lab but also the experience of researchers who used specific methodology, found a problem with that methodology in a new setting, and ultimately devised ways to minimize or obviate the limitations of the technology.

Keywords

Affinity chromatography Affinity electrophoresis Antibodies Antigen DNA Glucose Herman Organelle Polyacrylamid-Gelelektrophorese Protein avidin Vitamin biotin proteins

Editors and affiliations

  • Robert J. McMahon
    • 1
  1. 1.Mead Johnson NutritionalsEvansville

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-579-4
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-583-5
  • Online ISBN 978-1-59745-579-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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