SUMO Protocols

  • Helle D. Ulrich

Part of the METHODS IN MOLECULAR BIOLOGY™ book series (MIMB, volume 497)

Table of contents

  1. Front Matter
    Pages i-x
  2. Introduction

    1. Helle D. Ulrich
      Pages 3-16
  3. Identification of Sumo Targets

  4. Identification Of Sumo Interactors

    1. Mary B. Kroetz, Mark Hochstrasser
      Pages 107-120
    2. Candace S. Seu, Yuan Chen
      Pages 121-138
  5. Functional Analysis Of Sumoylation In Vivo

  6. Biochemical Studies Of Sumo Conjugation

    1. Andreas Werner, Marie-Christine Moutty, Ulrike Möller, Frauke Melchior
      Pages 187-199
    2. Puck Knipscheer, Helene Klug, Titia K. Sixma, Andrea Pichler
      Pages 201-210
    3. Hisato Saitoh, Junsuke Uwada, Kawasaki Azusa
      Pages 211-221
  7. Biochemical Studies of Sumo Deconjugation

    1. David Reverter, Christopher D. Lima
      Pages 225-239
    2. Nicolas Stankovic-Valentin, Lukasz Kozaczkiewicz, Katja Curth, Frauke Melchior
      Pages 241-251
    3. Michael H. Tatham, Ronald T. Hay
      Pages 253-268
    4. Craig A. Leach, Xufan Tian, Michael R. Mattern, Benjamin Nicholson
      Pages 269-281
  8. Manipulation And Alternative Uses Of The Sumo System

    1. Mariaelena Pozzebon, Chiara V. Segré, Susanna Chiocca
      Pages 285-301

About this book

Introduction

While the small ubiquitin-related modifier SUMO uses a conjugation and de-conjugation system closely related to that of ubiquitin itself, its functions are highly diverse and largely independent of the ubiquitin system. In SUMO Protocols, leading experts present a collection of methods intended to highlight important features unique to the biology of SUMO in order to support future SUMO research. The first half of the volume focuses on the identification and functional characterization of SUMO targets and interactors, while the second half covers essential biochemical methods for the manipulation of the enzymatic components of the SUMO system. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and the Notes sections, which provide tips on troubleshooting and avoiding known pitfalls.


Unique and cutting-edge, SUMO Protocols provides the necessary tools for biochemists, molecular and cellular biologists as well as research-oriented clinicians not yet familiar with this vital system.

Keywords

Escherichia coli Proteomics Vivo enzymes molecular biology regulation saccharomyces cerevisiae transcription

Editors and affiliations

  • Helle D. Ulrich
    • 1
  1. 1.London Research Institute Clare Hall LaboratoriesCancer Research UKHertsUK

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-566-4
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-934115-80-0
  • Online ISBN 978-1-59745-566-4
  • Series Print ISSN 1064-3745
  • About this book
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