DNA Methylation

Methods and Protocols

  • Jörg Tost

Part of the Methods in Molecular Biology book series (MIMB, volume 507)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Introduction

  3. Global Methylation Levels

    1. Front Matter
      Pages 21-21
    2. Mar’a Berdasco, Mario F Fraga, Manel Esteller
      Pages 23-34
    3. Kenneth P Nephew, Curt Balch, David G Skalnik
      Pages 35-41
  4. Methods For Whole Genome Analysis Of Dna Methylation Patterns

    1. Front Matter
      Pages 43-43
    2. Johannes Pröll, Christian Wechselberger, Mathilde Födermayr, Otto Zach, Dieter Lutz
      Pages 45-54
    3. Fabio Mohn, Michael Weber, Dirk Schübeler, Tim-Christoph Roloff
      Pages 55-64
    4. Tibor A. Rauch, Gerd P. Pfeifer
      Pages 65-75
    5. Mayumi Oda, John M. Greally
      Pages 77-87
    6. Pearlly S. Yan, Dustin Potter, Daniel E. Deatherage, Tim H.-M. Huang, Shili Lin
      Pages 89-106
    7. Mireia Jordà, Jairo Rodríguez, Jordi Frigola, Miguel A. Peinado
      Pages 107-116
    8. Toshikazu Ushijima, Satoshi Yamashita
      Pages 117-130
    9. Joseph F. Costello, Chibo Hong, Christoph Plass, Dominic J. Smiraglia
      Pages 131-148
    10. Marina Bibikova, Jian-Bing Fan
      Pages 149-163
    11. Paul Cairns
      Pages 165-174
  5. Gene-Specific Methylation Analysis

    1. Front Matter
      Pages 175-175
    2. Yingying Zhang, Christian Rohde, Sascha Tierling, Heinrich Stamerjohanns, Richard Reinhardt, Jörn Walter et al.
      Pages 177-187
    3. Emelyne Dejeux, Hafida El abdalaoui, Ivo Glynne Gut, Jörg Tost
      Pages 189-205

About this book

Introduction

Over the past few years, DNA methylation technologies and our knowledge of DNA methylation patterns have been advancing at a breathtaking pace.  Due to this fact, DNA Methylation: Methods and Protocols, Second Edition completely revises, updates, and expands upon the popular first edition with the most current novel techniques, easier to use and more refined by the tested experience of leading experts.  This revision reflects contemporary study of the subject: the analysis of gene-specific DNA methylation patterns has been complemented by genome-wide approaches, and epigenomics takes a central place. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume present brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which highlight tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, DNA Methylation: Methods and Protocols, Second Edition is the perfect resource for scientists who wish to further our understanding of the molecular processes that determine the genomic DNA methylation landscape and for those who aim to identify and implement DNA methylation-based biomarkers for clinical and diagnostic applications.

Keywords

DNA Epigenetics Epigenomics Gene-specific methylation Microarray PCR Termination Whole genome analysis genes hybridization molecular biology

Editors and affiliations

  • Jörg Tost
    • 1
  1. 1.CEA - Institut de GénomiqueEvryFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-522-0
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-934115-61-9
  • Online ISBN 978-1-59745-522-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
Industry Sectors
Biotechnology
Consumer Packaged Goods
Pharma