Protein Targeting Protocols

  • Mark van der Giezen

Part of the Methods in Molecular Biology™ book series (MIMB, volume 390)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Bacterial Systems

    1. Front Matter
      Pages 1-1
    2. Jeanine de Keyzer, Martin van der Laan, Arnold J.M. Driessen
      Pages 17-32
    3. Filipe J. Mergulhão, Gabriel A. Monteiro
      Pages 47-61
    4. Michael Moser, Sascha Panahandeh, Eva Holzapfel, Matthias Müller
      Pages 63-79
  3. Eukaryotic Systems

    1. Front Matter
      Pages 81-81
    2. Jade K. Forwood, Gurpreet Kaur, David A. Jans
      Pages 83-97
    3. Kipros Gabriel, Nikolaus Pfanner
      Pages 99-117
    4. Shashi Bhushan, Pavel F. Pavlov, Charlotta Rudhe, Elzbieta Glaser
      Pages 131-150
    5. Panagiotis Papatheodorou, Grażyna Domańska, Joachim Rassow
      Pages 151-166
    6. Friederike Hörmann, Jürgen Soll, Bettina Bölter
      Pages 179-193
    7. Bettina Bölter, Jürgen Soll
      Pages 195-206
    8. Rostislav Vacula, Silvia Slàvikovà, Steven D. Schwartzbach
      Pages 219-237
    9. John Runions, Chis Hawes, Smita Kurup
      Pages 239-256
    10. Peter G. Kroth
      Pages 257-267

About this book


In this highly anticipated update of the extremely successful Protein Targeting Protocols, experts from around the world provide the latest protocols on for isolating different organelles and the localization of particular proteins using a variety of methods such as light, confocal, and electron microscopy. Unlike the first edition, however, this volume places emphasis on protein targeting of cellular compartments in both prokaryotic and eukaryotic systems.

Authors provide state-of-the-art information on the fast moving fields of import machinery of mitochondria and plastids, as well as a detailed protocol on the movement of protein complexes in bacterial membranes using fluorescent recovery after photobleaching. Bacterial protein targeting protocols using the Sec-system, type-V secretion apparatus, and the Tat-pathway are described, as is a periplasmic targeting protocol. Chapters also include bioinformatics methods to guide readers through the ever-increasing number of in silico tools currently available. Editor Mark van der Giezen has succeeded in including targeting protocols from different systems, including animal, plant, fungal, and protist models, providing insights into the intricate challenges presented by these diverse systems.

Authoritative and state-of-the-art, Protein Targeting Protocols, Second Edition, provides useful techniques that will aid scientists working with a variety of organisms.


Escherichia coli Organelle Polyacrylamid-Gelelektrophorese bioinformatics electron microscopy genes membrane proteins microscopy protein complexes saccharomyces cerevisiae

Editors and affiliations

  • Mark van der Giezen
    • 1
  1. 1.School of Biological and Chemical SciencesQueen Mary, University of LondonUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-702-0
  • Online ISBN 978-1-59745-466-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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