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  • © 2007

Mitochondria

Practical Protocols

  • Comprehensive lab manual for mitochondrial research
  • Introduction to different model organisms for studying mitochondrial function
  • Fusion and fission of mitochondria
  • Biogenesis of mitochondria
  • Detailed overview of functional genomic approaches for studying mitochondrial function

Part of the book series: Methods in Molecular Biology (MIMB, volume 372)

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Table of contents (39 protocols)

  1. Biochemistry

    1. The Analysis of tRNA Import Into Mammalian Mitochondria

      • Anne-Marie Mager-Heckel, Nina Entelis, Irina Brandina, Petr Kamenski, Igor A. Krasheninnikov, Robert P. Martin et al.
      Pages 235-253
    2. Analysis of Mitochondrial Protein Synthesis in Yeast

      • Soledad Funes, Johannes M. Herrmann
      Pages 255-263
    3. Protein Import Into Isolated Mitochondria

      • Dejana Mokranjac, Walter Neupert
      Pages 277-286
    4. In Vitro and In Vivo Protein Import Into Plant Mitochondria

      • Pavel F. Pavlov, Charlotta Rudhe, Shashi Bhushan, Elzbieta Glaser
      Pages 297-314
    5. Investigation of Iron-Sulfur Protein Maturation in Eukaryotes

      • Oliver Stehling, Paul M. Smith, Annette Biederbick, Janneke Balk, Roland Lill, Ulrich Mühlenhoff
      Pages 325-342
    6. Studying Proteolysis Within Mitochondria

      • Takashi Tatsuta, Thomas Langer
      Pages 343-360
  2. Physiology

    1. Front Matter

      Pages 361-361
    2. Methods to Determine the Status of Mitochondrial ATP Synthase Assembly

      • Sharon H. Ackerman, Alexander Tzagoloff
      Pages 363-377
    3. ATP Production in Isolated Mitochondria of Procyclic Trypanosoma brucei

      • André Schneider, Nabile Bouzaidi-Tiali, Anne-Laure Chanez, Laurence Bulliard
      Pages 379-387
    4. Oxidative Stress and Plant Mitochondria

      • Nicolas L. Taylor, A. Harvey Millar
      Pages 389-403
  3. Dynamics and Morphology

    1. Front Matter

      Pages 431-431
    2. Fluorescence Imaging of Mitochondria in Yeast

      • Theresa C. Swayne, Anna C. Gay, Liza A. Pon
      Pages 433-459
    3. Mitochondrial Fusion In Vitro

      • Shelly L. Meeusen, Jodi Nunnari
      Pages 461-466
    4. Conventional and Immunoelectron Microscopy of Mitochondria

      • Edward M. Perkins, J. Michael McCaffery
      Pages 467-483
    5. Mitochondrial Division in Caenorhabditis elegans

      • Shilpa Gandre, Alexander M. van der Bliek
      Pages 485-501

About this book

Mitochondria: Practical Protocols offers a broad collection of methods for studying the molecular biology, function, and features of mitochondria. In the past decade, mitochondrial research has elucidated the important influence of mitochondrial processes on integral cell processes such as apoptosis and cellular aging. This practical guide presents a wide spectrum of mitochondrial methods, each written by specialists with solid experience and intended for implementation by novice and expert researchers alike.

Part I introduces major experimental model systems and discusses their specific advantages and limitations for functional analysis of mitochondria. The concise overview of general properties of mitochondrial systems is supplemented by detailed protocols for cultivation of model organisms. Parts II-VI comprise a robust collection of protocols for studying different molecular aspects of mitochondrial functions including: genetics and microbiology, biochemistry, physiology, dynamics and morphology, and functional genomics. Emphasis is placed on new and emerging topics in mitochondrial study, such as the examination of apoptotic effects, fusion and fission of mitochondria, and proteome and transcriptome analysis.

Editors and Affiliations

  • Department für Biologie I, Botanik, Ludwig-Maximilians-Universität München, München, Germany

    Dario Leister

  • Zellbiologie, Universität Kaiserslautern, Kaiserslautern, Germany

    Johannes M. Herrmann

Bibliographic Information

  • Book Title: Mitochondria

  • Book Subtitle: Practical Protocols

  • Editors: Dario Leister, Johannes M. Herrmann

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-365-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-667-2Published: 12 June 2007

  • Softcover ISBN: 978-1-62703-833-1Published: 26 November 2014

  • eBook ISBN: 978-1-59745-365-3Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 586

  • Topics: Cell Biology, Human Genetics

  • Industry Sectors: Biotechnology, Pharma

Buy it now

Buying options

eBook USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access