RNA

1. RNP Methods

   1. Isolation of RNP Granules

      • Lars Jønson, Finn Cilius Nielsen, Jan Christiansen

      Pages 265-273

   2. Electrophoretic Mobility Shift Assay for Characterizing RNA–Protein Interaction

      • Keith T. Gagnon, E. Stuart Maxwell

      Pages 275-291

   3. Polysome Analysis and RNA Purification from Sucrose Gradients

      • Tomáš Mašek, Leoš Valášek, Martin Pospíšek

      Pages 293-309

   4. Prediction of Targets for MicroRNAs

      • Morten Lindow

      Pages 311-317

   5. Outsourcing of Experimental Work

      • Henrik Nielsen

      Pages 319-325

2. Back Matter

   Pages 327-329

   PDF

Recent insight into the transcripts generated from the mammalian genome (i.e. the transcriptome) has revealed that transcription is a far more complex phenomenon than previously thought. In RNA: Methods and Protocols, expert researchers provide the procedures and methods used to describe the structure of messenger RNAs and non-coding RNAs that are transcribed by RNA polymerase II as the immediate gene products in mammalian cells. Focused on the structure of the RNA products of “gene X” and the mapping of proteins associated with these RNAs, the volume presents appropriate information for non-specialists in RNA biology. Written in the highly successful Methods in Molecular Biology™ series format, many chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, RNA: Methods and Protocols views the transcriptional landscape with an appreciation for the role that proteins play in the processing and interpretation of genetic information in an attempt to further our crucial knowledge of the many products and sophisticated regulatory networks that result from it.

• Bioinformatics
• Mammalian genome
• Messenger RNAs
• Microarray analysis
• Non-coding RNAs
• Protein mapping
• RNA polymerase II
• RNP methods
• Transcriptional landscape
• Transcriptome
• miRNA profiling
• gene expression

• The Panum Institute, Dept. of Cellular & Molecular Medicine, University of Copenhagen, Copenhagen, Denmark

  Henrik Nielsen

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