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  • © 2008

Plant Virology Protocols

From Viral Sequence to Protein Function

  • Offers step by step reproducible laboratory protocols written by experts in the field of plant sciences, virology, and genomics
  • Focus on the novel and emerging techniques used to analyze, understand, and treat plant viruses
  • Covers the spectrum of plant virology protocols, from general introductions to typical plant viral proteins, techniques for investigating viral acid sequences, through the emerging area of genomics and hist factor interactions
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 451)

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Table of contents (44 protocols)

  1. Protein Analysis and Investigation of Protein Function

    1. Movement Profiles: A Tool for Quantitative Analysis of Cell-to-Cell Movement of Plant Viral Movement Proteins

      • Kateryna Trutnyeva, Pia Ruggenthaler, Elisabeth Waigmann
      Pages 317-329
    2. Analysis of siRNA-Suppressor of Gene Silencing Interactions

      • Lóránt Lakatos, József Burgyán
      Pages 331-337
    3. Phosphorylation Analysis of Plant Viral Proteins

      • Kristiina M. Mäkinen, Konstantin I. Ivanov
      Pages 339-359
    4. Membrane and Protein Dynamics in Virus-Infected Plant Cells

      • Michael Goodin, Romit Chakrabarty, Sharon Yelton
      Pages 377-393
    5. Site-Directed Mutagenesis of Whole Viral Genomes

      • Li Liu, George P. Lomonossoff
      Pages 395-404
    6. Protein–Protein Interactions: The Yeast Two-Hybrid System

      • Deyin Guo, Minna-Liisa Rajamäki, Jari Valkonen
      Pages 421-439
    7. NMR Analysis of Viral Protein Structures

      • Andrew J. Dingley, Inken Lorenzen, Joachim Grötzinger
      Pages 441-462
    8. Localization of Viral Proteins in Plant Cells: Protein Tagging

      • Sophie Haupt, Angelika Ziegler, Lesley Torrance
      Pages 463-473
  2. Microscopy/GFP/Protein Tagging/Infections Clones and Other Such Tools

    1. Insertion of Introns: A Strategy to Facilitate Assembly of Infectious Full Length Clones

      • I. Elisabeth Johansen, Ole Søgaard Lund
      Pages 535-544
    2. Agroinoculation: A Simple Procedure for Systemic Infection of Plants with Viruses

      • Zarir E. Vaghchhipawala, Kirankumar S. Mysore
      Pages 555-562
    3. Geminivirus: Biolistic Inoculation and Molecular Diagnosis

      • Anésia A. Santos, Lilian H. Florentino, Acássia B. L. Pires, Elizabeth P. B. Fontes
      Pages 563-579

About this book

Following the considerable success of the first edition of Plant Virology Protocols, this exciting new edition covers the many new techniques that are now applied to the examination and understanding of plant viruses. Each section of Plant Virology Protocols, Second Edition presents the most novel methods and protocols to allow researchers more effective approaches to study plant viruses. The many subjects covered in this important new edition range from techniques for investigating viral nucleic acid sequence, protein analysis and investigation of protein function, techniques for microscopy/GFP visualization and analysis/protein tagging/generation of infectious clones and other such tools, as well as areas of genomics and interactions with host factors and plant-based studies. Plant Virology Protocols, Second Edition: From viral sequence to protein function is indispensable to laboratory investigators studying plant viruses.

Reviews

From the reviews of the second edition:

"This book describes a wide range of techniques on the molecular analyses of plant viruses and some of their interactions with their hosts. Many techniques could also be of interest to scientists studying animal and bacterial viruses. … this book should be of great use to virology researchers who take the correct approach." (Roger Hull, Microbiology Today, February, 2009)

Editors and Affiliations

  • University of Bristol, Bristol, UK

    Gary D. Foster

  • University of Aarhus, Aarhus, Denmark

    I. Elisabeth Johansen

  • University of Warwick, Warwick, UK

    Yiguo Hong

  • University of Kentucky, Kentucky, USA

    Peter D. Nagy

Bibliographic Information

  • Book Title: Plant Virology Protocols

  • Book Subtitle: From Viral Sequence to Protein Function

  • Editors: Gary D. Foster, I. Elisabeth Johansen, Yiguo Hong, Peter D. Nagy

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-102-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2008

  • Hardcover ISBN: 978-1-58829-827-0Published: 07 March 2008

  • Softcover ISBN: 978-1-61737-794-5Published: 10 December 2010

  • eBook ISBN: 978-1-59745-102-4Published: 16 March 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XX, 677

  • Number of Illustrations: 61 b/w illustrations, 1 illustrations in colour

  • Topics: Plant Sciences, Virology, Plant Genetics and Genomics

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access