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Xenopus Protocols

Cell Biology and Signal Transduction

  • Book
  • © 2006

Overview

Editors:
  1. X. Johné Liu

    1. Ottawa Health Research Institute, Ottawa Hospital, Ottawa, Canada

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Part of the book series: Methods in Molecular Biology (MIMB, volume 322)

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Resources for Genetic and Genomic Studies of Xenopus

    • Steven L. Klein, Daniela S. Gerhard, Lukas Wagner, Paul Richardson, Lynn M. Schriml, Amy K. Sater et al.
    Pages 1-16

  3. The Physiology of the Xenopus laevis Ovary

    • Melissa A. Rasar, Stephen R. Hammes
    Pages 17-30

  4. Oocyte Isolation and Enucleation

    • X. Shawn Liu, X. Johné Liu
    Pages 31-41

  5. Xenopus tropicalis Oocytes

    • Jean-François L. Bodart, Nicholas S. Duesbery
    Pages 43-53

  6. Oocyte Expression With Injection of Purified T7 RNA Polymerase

    • Xavier Altafaj, Nathalie Joux, Michel Ronjat, Michel De Waard
    Pages 55-67

  7. Visualization of the Cytoskeleton in Xenopus Oocytes and Eggs by Confocal Immunofluorescence Microscopy

    • Bret E. Becker, David L. Gard
    Pages 69-86

  8. Multiphoton Laser Scanning Microscopy as a Tool for Xenopus Oocyte Research

    • Angela M. Prouty, Jun Wu, Da-Ting Lin, Patricia Camacho, James D. Lechleiter
    Pages 87-101

  9. Imaging Ca2+ Signals in Xenopus Oocytes

    • Sheila L. Dargan, Angelo Demuro, Ian Parker
    Pages 103-119

  10. Chromosomal DNA Replication in a Soluble Cell-Free System Derived From Xenopus Eggs

    • Antonin V. Tutter, Johannes C. Walter
    Pages 121-137

  11. Chromatin Assembly of DNA Templates Microinjected Into Xenopus Oocytes

    • Danièle Roche, Geneviève Almouzni, Jean-Pierre Quivy
    Pages 139-147

  12. Pre-mRNA Splicing in the Nuclei of Xenopus Oocytes

    • Kyong Hwa Moon, Xinliang Zhao, Yi-Tao Yu
    Pages 149-163

  13. Chromatin Immunoprecipitation for Studying Transcriptional Regulation in Xenopus Oocytes and Tadpoles

    • David Stewart, Akihiro Tomita, Yun-Bo Shi, Jiemin Wong
    Pages 165-181

  14. Cytoplasmic mRNA Polyadenylation and Translation Assays

    • Maria Piqué, José Manuel López, Raúl Méndez
    Pages 183-198

  15. Xenopus Egg Extracts

    • Kathleen I. J. Sherman
    Pages 199-212

  16. Analysis of Molecular Chaperones Using a Xenopus Oocyte Protein Refolding Assay

    • John J. Heikkila, Angelo Kaldis, Rashid Abdulle
    Pages 213-222

  17. Ubiquitin-Mediated Protein Degradation in Xenopus Egg Extracts

    • Anna Castro, Suzanne Vigneron, Cyril Bernis, Jean-Claude Labbé, Thierry Lorca
    Pages 223-234

  18. Introduction to Nucleocytoplasmic Transport

    • Reiner Peters
    Pages 235-258

  19. Use of Xenopus laevis Oocyte Nuclei and Nuclear Envelopes in Nucleocytoplasmic Transport Studies

    • Reiner Peters
    Pages 259-272

  20. Nuclear Pore Complex Structure and Plasticity Revealed by Electron and Atomic Force Microscopy

    • Bohumil Maco, Birthe Fahrenkrog, Ning-Ping Huang, Ueli Aebi
    Pages 273-288
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Keywords

About this book

A collection of standard and cutting-edge techniques for using Xenopus oocytes and oocytes/egg extracts to reconstitute biological and cellular processes. These readily reproducible methods take advantage of the oocyte's impressive protein abundance, its striking protein translation capacity, and its breathtaking possibilities for the assembly of infectious viral particles by single cell injection of multiple RNAs. The authors focus on the versatility of frog oocytes and egg extracts in cell biology and signal transduction, and cover all the major uses of oocytes/extracts as experimental models.

Editors and Affiliations

  • Ottawa Health Research Institute, Ottawa Hospital, Ottawa, Canada

    X. Johné Liu

Bibliographic Information

  • Book Title: Xenopus Protocols

  • Book Subtitle: Cell Biology and Signal Transduction

  • Editors: X. Johné Liu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-000-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-362-6Published: 13 December 2005

  • Softcover ISBN: 978-1-61737-520-0Published: 19 November 2010

  • eBook ISBN: 978-1-59745-000-3Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 489

  • Topics: Biochemistry, general, Developmental Biology

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

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