Overview
- Editors:
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Caroline A. Evans
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Department of Chemical and Biological Engineering, University of Sheffield, Sheffield, UK
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Phillip C. Wright
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Faculty of Science, Agriculture & Engineering, Newcastle University, Newcastle upon Tyne, UK
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Josselin Noirel
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Laboratoire GBCM (EA4627), Conservatoire National des Arts et Métiers, HESAM Université, Paris, France
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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Table of contents (16 protocols)
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Front Matter
Pages i-xiii
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New Biological Insights from Technological Breakthroughs
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- Qifeng Lin, Hwee Tong Tan, Maxey C. M. Chung
Pages 3-15
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- Navin Chicooree, John R. Griffiths
Pages 17-24
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- Joseph Longworth, Gunnar Dittmar
Pages 25-34
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- Martin E. Barrios-Llerena, Thierry Le Bihan
Pages 35-42
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- Peder J. Lund, Yekaterina Kori, Xiaolu Zhao, Simone Sidoli, Zuo-Fei Yuan, Benjamin A. Garcia
Pages 43-70
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- Keith T. Woodley, Mark O. Collins
Pages 71-82
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- Paola Antinori, Théo Michelot, Pierre Lescuyer, Markus Müller, Adelina E. Acosta-Martin
Pages 99-113
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- Joel D. Federspiel, Ileana M. Cristea
Pages 115-143
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- David A. Russo, Narciso Couto, Andrew P. Beckerman, Jagroop Pandhal
Pages 145-155
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Dealing with Proteomics Data in a Big Data Era
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Front Matter
Pages 157-157
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- Martina Fischer, Thilo Muth, Bernhard Y. Renard
Pages 159-180
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- Tomasz Burzykowski, Jürgen Claesen, Dirk Valkenborg
Pages 181-197
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- Dana Pascovici, Xiaomin Song, Jemma Wu, Thiri Zaw, Mark Molloy
Pages 199-215
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- Andrew F. Jarnuczak, Tobias Ternent, Juan Antonio VizcaÃno
Pages 217-235
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- Paul A. Stewart, Brent M. Kuenzi, Subina Mehta, Praveen Kumar, James E. Johnson, Pratik Jagtap et al.
Pages 249-261
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Back Matter
Pages 263-264
About this book
New insights into modern medicine and systems biology are enabled by innovative protocols and advanced technologies in mass spectrometry-based proteomics. This volume details new pipelines, workflows, and ways to process data that allow for new frontiers in proteomics to be pushed forward. With applications to biomarker discovery, interactions between proteins, between biological systems, dynamics of post-translational modifications among others, new protocols have been developed and iteratively refined to probe the endless complexity of the proteome in ever greater details. This volume deals with methods for data dependent and data independent mass spectrometry analyses. Valuable, first-hand information is provided from designing experiments, sample preparation and analysis, exploitation of public datasets and carrying out reproducible data pipelines, using modern computational tools such as Galaxy or Jupyter. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mass Spectrometry of Proteins: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Editors and Affiliations
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Department of Chemical and Biological Engineering, University of Sheffield, Sheffield, UK
Caroline A. Evans
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Faculty of Science, Agriculture & Engineering, Newcastle University, Newcastle upon Tyne, UK
Phillip C. Wright
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Laboratoire GBCM (EA4627), Conservatoire National des Arts et Métiers, HESAM Université, Paris, France
Josselin Noirel
