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Target Identification and Validation in Drug Discovery

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Jürgen Moll

    1. Pharmacology and Translational Research, Boehringer Ingelheim RCV GmbH & Co KG, Vienna, Austria

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    You can also search for this editor in PubMed   Google Scholar

  2. Sebastian Carotta

    1. Department of Cancer Cell Signaling, Boehringer Ingelheim RCV GmBH & Co KG, Vienna, Austria

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1953)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Identification of Novel Targets Using High Throughput Screening Assays

    1. Front Matter

      Pages 1-1
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    2. Using Functional Genetics in Haploid Cells for Drug Target Identification

      • Jennifer C. Volz, Nicole Schuller, Ulrich Elling
      Pages 3-21

    3. Unbiased Forward Genetic Screening with Chemical Mutagenesis to Uncover Drug–Target Interactions

      • Moritz Horn, Franziska Metge, Martin S. Denzel
      Pages 23-31

    4. Integration of RNAi and Small Molecule Screens to Identify Targets for Drug Development

      • Konstantinos Drosopoulos, Spiros Linardopoulos
      Pages 33-42

    5. CellProfiler and KNIME: Open-Source Tools for High-Content Screening

      • Martin Stöter, Antje Janosch, Rico Barsacchi, Marc Bickle
      Pages 43-60

  3. Drug Target Profiling and Validation

    1. Front Matter

      Pages 61-61
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    2. In Silico Target Druggability Assessment: From Structural to Systemic Approaches

      • Jean-Yves Trosset, Christian Cavé
      Pages 63-88

    3. In Silico Drug–Target Profiling

      • Jean-Yves Trosset, Christian Cavé
      Pages 89-103

    4. Locus-Specific Knock-In of a Degradable Tag for Target Validation Studies

      • Matthias Brand, Georg E. Winter
      Pages 105-119

    5. Expression of Human VH Single Domains as Fc Fusions in Mammalian Cells

      • Mahmoud Abdelatti, Peter Schofield, Daniel Christ
      Pages 121-136

  4. Three Dimensional Cell Culture Techniques Mimicking the Tumor Microenvironment In Vitro

    1. Front Matter

      Pages 137-137
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    2. The Neurosphere Assay (NSA) Applied to Neural Stem Cells (NSCs) and Cancer Stem Cells (CSCs)

      • Rossella Galli
      Pages 139-149

    3. 3D-3 Tumor Models in Drug Discovery for Analysis of Immune Cell Infiltration

      • Annika Osswald, Viola Hedrich, Wolfgang Sommergruber
      Pages 151-162

    4. Establishment and Analysis of a 3D Co-Culture Spheroid Model of Pancreatic Adenocarcinoma for Application in Drug Discovery

      • Julia C. Meier-Hubberten, Michael P. Sanderson
      Pages 163-179

  5. Animal Models for the Study of Gene Function In Vivo

    1. Front Matter

      Pages 181-181
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    2. In Vivo Pharmacology Models for Cancer Target Research

      • Dawei Chen, Xiaoyu An, Xuesong Ouyang, Jie Cai, Demin Zhou, Qi-Xiang Li
      Pages 183-211

    3. Use of CRISPR/Cas9 for the Modification of the Mouse Genome

      • Alexander Klimke, Steffen Güttler, Petric Kuballa, Simone Janzen, Sonja Ortmann, Adriano Flora
      Pages 213-230

    4. Assessment of Gene Function of Mouse Innate Lymphoid Cells for In Vivo Analysis Using Retroviral Transduction

      • Cyril Seillet, Gabrielle T. Belz
      Pages 231-240

    5. Creation of PDX-Bearing Humanized Mice to Study Immuno-oncology

      • Li-Chin Yao, Ken-Edwin Aryee, Mingshan Cheng, Pali Kaur, James G. Keck, Michael A. Brehm
      Pages 241-252
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Keywords

About this book

This second edition book explores breakthrough technologies in the field of drug target identification and validation. The volume emphasizes particularly revolutionary technologies, such as CRISPR-related screening, “big data,” and in silico approaches, as well as in vivo applications of CRISPR and best uses of animal models in drug development. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Fully updated and authoritative, Target Identification and Validation in Drug Discovery: Methods and Protocols, Second Edition is an ideal guide for molecular and cellular biologists, pharmacologists, pathologists, bioinformaticians, clinical researchers, or investigators, as well as experts in other fieldsthat need a quick overview of these state-of-the-art technologies.

Editors and Affiliations

  • Pharmacology and Translational Research, Boehringer Ingelheim RCV GmbH & Co KG, Vienna, Austria

    Jürgen Moll

  • Department of Cancer Cell Signaling, Boehringer Ingelheim RCV GmBH & Co KG, Vienna, Austria

    Sebastian Carotta

Bibliographic Information

  • Book Title: Target Identification and Validation in Drug Discovery

  • Book Subtitle: Methods and Protocols

  • Editors: Jürgen Moll, Sebastian Carotta

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9145-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9144-0Published: 26 March 2019

  • eBook ISBN: 978-1-4939-9145-7Published: 25 March 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 318

  • Number of Illustrations: 11 b/w illustrations, 29 illustrations in colour

  • Topics: Pharmaceutical Sciences/Technology

  • Industry Sectors: Biotechnology, Chemical Manufacturing, Consumer Packaged Goods, Pharma

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