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Plant Metabolomics

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Carla António

    1. Plant Metabolomics Laboratory, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), Oeiras, Portugal

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  • Includes cutting-edge methods and protocols for the study of plant metabolomics
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1778)

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Table of contents (24 protocols)

  1. Mass Spectrometry-Based Methodology Chapters

    1. Laser Ablation Electrospray Ionization-Mass Spectrometry Imaging (LAESI-MS) for Spatially Resolved Plant Metabolomics

      • Desalegn W. Etalo, Carmen Díez-Simón, Ric C. H. de Vos, Robert D. Hall
      Pages 253-267

    2. Live Single-Cell Mass Spectrometry (LSC-MS) for Plant Metabolomics

      • Keiko Masuda, Yasmine Abouleila, Ahmed Ali, Toshio Yanagida, Tsutomu Masujima
      Pages 269-282

  2. Data Analysis & Statistics Chapters

    1. Front Matter

      Pages 283-283
      Download chapter PDF

    2. Statistical and Multivariate Analysis of MS-Based Plant Metabolomics Data

      • Carsten Jaeger, Jan Lisec
      Pages 285-296

    3. Integration of Plant Metabolomics Data with Metabolic Networks: Progresses and Challenges

      • Nadine Töpfer, Samuel M. D. Seaver, Asaph Aharoni
      Pages 297-310

    4. Understanding the Functionality of a Biological System as a Whole: Comparative Data Analysis

      • Isabel Orf
      Pages 311-319

    5. Regression-Based Modeling of Complex Plant Traits Based on Metabolomics Data

      • Francisco de Abreu e Lima, Lydia Leifels, Zoran Nikoloski
      Pages 321-327

    6. Mathematical Modeling Approaches in Plant Metabolomics

      • Lisa Fürtauer, Jakob Weiszmann, Wolfram Weckwerth, Thomas Nägele
      Pages 329-347

  3. Back Matter

    Pages 349-355
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Keywords

About this book

This detailed book aims to address the need for standardizing key steps in plant metabolomics research that is still considered a critical issue in the field.  The collection unites diverse mass spectrometry (MS)-based protocols, from sample preparation and extraction of plant primary and secondary metabolites (e.g. a range of sugars, lipids, phytohormones and volatile organic compounds) to metabolite analysis, using exclusively highly sensitive MS-based methodologies, followed by frontline bioinformatics and/or mathematical modeling approaches to produce reliable biological data interpretation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Plant Metabolomics: Methods and Protocols serves both new and well-established researchers in the plant metabolomics field in finding helpful guidelines and useful laboratory-based protocols for setting up their routine plant metabolomics experiments.

Editors and Affiliations

  • Plant Metabolomics Laboratory, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), Oeiras, Portugal

    Carla António

Bibliographic Information

  • Book Title: Plant Metabolomics

  • Book Subtitle: Methods and Protocols

  • Editors: Carla António

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7819-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7818-2Published: 15 May 2018

  • Softcover ISBN: 978-1-4939-9294-2Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7819-9Published: 14 May 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 355

  • Number of Illustrations: 27 b/w illustrations, 51 illustrations in colour

  • Topics: Plant Sciences

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

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