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Bacterial Regulatory RNA

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1737)

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Table of contents (21 protocols)

  1. Cofactors of sRNA-Based Regulation

    1. Single-Molecule FRET Assay to Observe the Activity of Proteins Involved in RNA/RNA Annealing

      • Thierry Bizebard, Véronique Arluison, Ulrich Bockelmann
      Pages 301-319
    2. Techniques to Analyze sRNA Protein Cofactor Self-Assembly In Vitro

      • David Partouche, Antoine Malabirade, Thomas Bizien, Marisela Velez, Sylvain Trépout, Sergio Marco et al.
      Pages 321-340
    3. Sequence-Specific Affinity Chromatography of Bacterial Small Regulatory RNA-Binding Proteins from Bacterial Cells

      • Jonathan Gans, Jonathan Osborne, Juliet Cheng, Louise Djapgne, Amanda G. Oglesby-Sherrouse
      Pages 341-350
    4. Identification of Small RNA–Protein Partners in Plant Symbiotic Bacteria

      • Marta Robledo, Ana M. Matia-González, Natalia I. García-Tomsig, José I. Jiménez-Zurdo
      Pages 351-370
  2. Applications in Synthetic Biology

    1. Front Matter

      Pages 371-371
    2. A Modular Genetic System for High-Throughput Profiling and Engineering of Multi-Target Small RNAs

      • Samuel D. Stimple, Ashwin Lahiry, Joseph E. Taris, David W. Wood, Richard A. Lease
      Pages 373-391
  3. Back Matter

    Pages 393-395

About this book

This volume details the most important methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters present methods in sections covering different aspects of the biology of that field: identification of ncRNAs, their differential expression, characterization of their structure, abundance, intracellular location and function, their interaction with RNA binding proteins, and plausible applications of ncRNA elements in the rapidly emerging field of synthetic biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bacterial Regulatory RNA: Methods and Protocols serves as a guidebook for scientists working toward the development of new tools and proceduresfor the vital field of sRNA biology.

Editors and Affiliations

  • Université Paris Diderot & LLB/CEA/CNRS, Gif-sur-Yvette, France

    Véronique Arluison

  • Laboratorio de Bioquímica, Microbiología e Interacciones, Biológicas en el Suelo, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes-CONICET, Bernal, Argentina

    Claudio Valverde

Bibliographic Information

  • Book Title: Bacterial Regulatory RNA

  • Book Subtitle: Methods and Protocols

  • Editors: Véronique Arluison, Claudio Valverde

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7634-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7633-1Published: 27 February 2018

  • Softcover ISBN: 978-1-4939-9251-5Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7634-8Published: 26 February 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 395

  • Number of Illustrations: 22 b/w illustrations, 62 illustrations in colour

  • Topics: Human Genetics

  • Industry Sectors: Biotechnology, Pharma

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 139.00
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access