Bacterial Regulatory RNA

Methods and Protocols

  • Véronique Arluison
  • Claudio Valverde

Part of the Methods in Molecular Biology book series (MIMB, volume 1737)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Identification of sRNA

    1. Front Matter
      Pages 1-1
    2. Carl T. Fakhry, Kourosh Zarringhalam, Rahul V. Kulkarni
      Pages 47-56
  3. Function and Differential Expression of sRNA

    1. Front Matter
      Pages 57-57
    2. Alexander J. Westermann, Jörg Vogel
      Pages 59-75
    3. Saumya Jani, Alexis Jackson, Carol Davies-Sala, Kevin Chiem, Alfonso Soler-Bistué, Angeles Zorreguieta et al.
      Pages 89-98
    4. Cédric Nadiras, Annie Schwartz, Mildred Delaleau, Marc Boudvillain
      Pages 99-118
    5. Abigail N. Leistra, Mia K. Mihailovic, Lydia M. Contreras
      Pages 129-164
    6. Eva Maria Sternkopf Lillebæk, Birgitte Haahr Kallipolitis
      Pages 165-176
    7. Erich Michel, Olivier Duss, Frédéric H.-T. Allain
      Pages 177-195
  4. Quantitation and Subcellular Localisation of sRNA

    1. Front Matter
      Pages 197-197
    2. Seongjin Park, Magda Bujnowska, Eric L. McLean, Jingyi Fei
      Pages 199-212
    3. Janine Habier, Patrick May, Anna Heintz-Buschart, Anubrata Ghosal, Anke K. Wienecke-Baldacchino, Esther N. M. Nolte-‘t Hoen et al.
      Pages 213-230
    4. Florent Busi, Véronique Arluison, Philippe Régnier
      Pages 231-248
  5. Cofactors of sRNA-Based Regulation

    1. Front Matter
      Pages 249-249
    2. Brandon Sy, Julia Wong, Sander Granneman, David Tollervey, David Gally, Jai J. Tree
      Pages 251-272

About this book

Introduction

This volume details the most important methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters present methods in sections covering different aspects of the biology of that field: identification of ncRNAs, their differential expression, characterization of their structure, abundance, intracellular location and function, their interaction with RNA binding proteins, and plausible applications of ncRNA elements in the rapidly emerging field of synthetic biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bacterial Regulatory RNA: Methods and Protocols: Methods and Protocol aims to be a guidebook to scientists, hoping to lead to the development of new tools and procedures for further developments in the field of sRNA biology. 

Keywords

sRNA intracellular localization prokaryotic non-coding RNAs ncRNAs RNA binding proteins

Editors and affiliations

  • Véronique Arluison
    • 1
  • Claudio Valverde
    • 2
  1. 1.Université Paris Diderot & LLB/CEA/CNRSGif-sur-YvetteFrance
  2. 2.Laboratorio de Bioquímica, Microbiología e Interacciones, Biológicas en el Suelo, Departamento de Ciencia y TecnologíaUniversidad Nacional de Quilmes-CONICETBernalArgentina

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7634-8
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7633-1
  • Online ISBN 978-1-4939-7634-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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