DNA Topoisomerases

Methods and Protocols

  • Marc Drolet

Part of the Methods in Molecular Biology book series (MIMB, volume 1703)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Florence Garnier, Hélène Debat, Marc Nadal
    Pages 1-20
  3. Jang-Eun Cho, Sue Jinks-Robertson
    Pages 21-45
  4. Yingting Liu, Zhi-Chun Hua, Fenfei Leng
    Pages 63-73
  5. Alicia Castán, Pablo Hernández, Dora B. Krimer, Jorge B. Schvartzman
    Pages 75-86
  6. Hafez El Sayyed, Olivier Espéli
    Pages 87-94
  7. Fedor Kouzine, Laura Baranello, David Levens
    Pages 95-108
  8. Aziz El Hage, David Tollervey
    Pages 109-129
  9. Makisha Martel, Aurélien Balleydier, Julien Brochu, Marc Drolet
    Pages 131-138
  10. Kathryn H. Gunn, John F. Marko, Alfonso Mondragón
    Pages 139-152
  11. Shun-Hsiao Lee, Tao-shih Hsieh, Grace Ee-Lu Siaw
    Pages 153-159
  12. Muzammil Ahmad, Dongyi Xu, Weidong Wang
    Pages 161-172
  13. Shih-Chieh Chiang, Kirsty Liversidge, Sherif F. El-Khamisy
    Pages 173-181
  14. Kayleigh A. Smith, Ian G. Cowell, Caroline A. Austin
    Pages 183-189
  15. Juan F. Giménez-Abián, Andrew B. Lane, Duncan J. Clarke
    Pages 191-215
  16. Katherine Furniss, Amit C. J. Vas, Andrew B. Lane, Duncan J. Clarke
    Pages 217-240
  17. Jessica S. Williams, Thomas A. Kunkel
    Pages 241-257
  18. Thirunavukkarasu Annamalai, Bokun Cheng, Neelam Keswani, Yuk-Ching Tse-Dinh
    Pages 259-268
  19. Gan Luan, Karl Drlica
    Pages 269-281

About this book

Introduction

This volume looks at the evolution of the topo field over the last year and discusses how DNA topoisomerases are directly and indirectly involved in numerous cellular processes. The chapters in this book cover various topics, such as type 1 topos with an evolutionary perspective; antimicrobial agents targeting topos; experimental protocols related to DNA topology, including a basic technique to measure DNA supercoiling in bacteria; protocols for in vitro studies, including assays to detect RNA topo activity; topo inhibitors; and a study of plasmid-mediated quinolone resistance in bacteria. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, DNA Topoisomerases: Methods and Protoc

ols is intended for specialists and non-specialists with an overview of selected hot topics in the field, and the latest protocols to study DNA topology, topoisomerases functions and inhibition by various compounds.

Keywords

genome stability supercoiled DNA chromosome segregation Cre/loxP Purification topoisomerases

Editors and affiliations

  • Marc Drolet
    • 1
  1. 1.Département de microbiologie, infectiologie et immunologieUniversité de MontréalMontréalCanada

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7459-7
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7458-0
  • Online ISBN 978-1-4939-7459-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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