Plant Membrane Proteomics

Methods and Protocols

  • Hans-Peter Mock
  • Andrea Matros
  • Katja Witzel

Part of the Methods in Molecular Biology book series (MIMB, volume 1696)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Enni Väisänen, Junko Takahashi, Luis A. Jiménez Barboza, Xianbao Deng, Teemu H. Teeri, Kurt V. Fagerstedt et al.
    Pages 13-39
  3. Aarti Pandey, Subhra Chakraborty, Niranjan Chakraborty
    Pages 41-55
  4. Ivo Chamrád, Jana Uřinovská, Beáta Petrovská, Hana Jeřábková, René Lenobel, Jan Vrána et al.
    Pages 57-79
  5. Kazusato Oikawa, Takuya Inomata, Yoshitoshi Hirao, Tadashi Yamamoto, Marouane Baslam, Kentaro Kaneko et al.
    Pages 91-105
  6. Miwa Ohnishi, Katsuhisa Yoshida, Tetsuro Mimura
    Pages 107-116
  7. Roque Bru-Martínez, Ascensión Martínez-Márquez, Jaime Morante-Carriel, Susana Sellés-Marchart, María José Martínez-Esteso, José Luis Pineda-Lucas et al.
    Pages 147-162
  8. Christine Schikowsky, Beate Thal, Hans-Peter Braun, Holger Eubel
    Pages 163-183
  9. Anna M. Jozefowicz, Andrea Matros, Katja Witzel, Hans-Peter Mock
    Pages 195-204
  10. Małgorzata Janicka, Anna Wdowikowska, Grażyna Kłobus
    Pages 205-215
  11. Anja Hartmann, Anna Maria Jozefowicz
    Pages 261-278
  12. Back Matter
    Pages 279-280

About this book

Introduction

This volume provides comprehensive and detailed  protocols that discuss proteomic techniques, plant endosomes, and isolation of organelles and subcellular fractions. The chapters in this book explore numerous plant species and cover topics, such as isolation and purity assessment of membranes from Norway spruce; proteomic analysis of nuclei; analyzing the vacuolar membrane (tonoplast) proteome; isoforms of a thylakoid-bound protein; assay of plasma membrane H+-ATPase in plant tissue under abiotic stresses; and identification and characterization of plant membrane proteins using ARAMEMNON. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, Plant Membrane Proteomics: Methods and Protocols is a valuable resource that promotes the use of plant membrane proteomics to develop the future of the field.

Keywords

2-D gel electrophoresis liquid chromatography (LC)-based peptide separation mass spectrometry (MS) plasma membranes plant cellular networks Arabidopsis subcellular fractionation

Editors and affiliations

  • Hans-Peter Mock
    • 1
  • Andrea Matros
    • 2
  • Katja Witzel
    • 3
  1. 1.Leibniz Institute of Plant Genetics and Crop Plant ResearchGaterslebenGermany
  2. 2.Leibniz Institute of Plant Genetics and Crop Plant ResearchGaterslebenGermany
  3. 3.Leibniz Institute of Vegetable and Ornamental CropsGroßbeerenGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7411-5
  • Copyright Information Springer Science+Business Media LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7409-2
  • Online ISBN 978-1-4939-7411-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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