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Recombinant Glycoprotein Production

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Virgínia Picanço-Castro

    1. Regional Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil

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    You can also search for this editor in PubMed   Google Scholar

  2. Kamilla Swiech

    1. School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1674)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Platforms for Recombinant Therapeutic Glycoprotein Production

    • Amanda Mizukami, Angelo Luis Caron, Virgínia Picanço-Castro, Kamilla Swiech
    Pages 1-14

  3. Uncovering Innovation Features and Emerging Technologies in Molecular Biology through Patent Analysis

    • Cristiano Gonçalves Pereira, Geciane Silveira Porto
    Pages 15-34

  4. Upstream Processes

    1. Front Matter

      Pages 35-35
      Download chapter PDF

    2. Production of Full-Length Antibody by Pichia pastoris

      • Adam Nylen, Ming-Tang Chen
      Pages 37-48

    3. Human Cells as Platform to Produce Gamma-Carboxylated Proteins

      • Aline de Sousa Bomfim, Marcela Cristina Corrêa de Freitas, Dimas Tadeu Covas, Elisa Maria de Sousa Russo
      Pages 49-61

    4. Production of Recombinant Factor VIII in Human Cell Lines

      • Daianne Maciely Carvalho Fantacini, Virgínia Picanço-Castro
      Pages 63-74

    5. Strategies to Suspension Serum-Free Adaptation of Mammalian Cell Lines for Recombinant Glycoprotein Production

      • Angelo Luis Caron, Rafael Tagé Biaggio, Kamilla Swiech
      Pages 75-85

    6. Production of Recombinant Rabies Virus Glycoprotein by Insect Cells in a Single-Use Fixed-Bed Bioreactor

      • Daniella C. Ventini-Monteiro, Renato M. Astray, Carlos A. Pereira
      Pages 87-94

    7. Cell-Free Production of Protein Biologics Within 24 H

      • Challise J. Sullivan, Erik D. Pendleton, John Dresios
      Pages 95-107

    8. Demonstration-Scale High-Cell-Density Fermentation of Pichia pastoris

      • Wan-Cang Liu, Ping Zhu
      Pages 109-116

    9. Large-Scale Transient Transfection of Suspension Mammalian Cells for VLP Production

      • Laura Cervera, Amine A. Kamen
      Pages 117-127

    10. Bioreactor-Based Production of Glycoproteins in Plant Cell Suspension Cultures

      • Tanja Holland, Johannes Felix Buyel
      Pages 129-146

    11. Fed-Batch CHO Cell Culture for Lab-Scale Antibody Production

      • Yuzhou Fan, Daniel Ley, Mikael Rørdam Andersen
      Pages 147-161

    12. Strategies to Develop Therapeutic N- and O-Hyperglycosylated Proteins

      • Agustina Gugliotta, Natalia Ceaglio, Marina Etcheverrigaray, Ricardo Kratje, Marcos Oggero
      Pages 163-181

    13. Expression of Glycosylated Proteins in Bacterial System and Purification by Affinity Chromatography

      • Carlos Alexandre Breyer, Marcos Antonio de Oliveira, Adalberto Pessoa Jr
      Pages 183-191

  5. Downstream Processes

    1. Front Matter

      Pages 193-193
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    2. Purification Methods for Recombinant Factor VIII Expressed in Human Liver SK-Hep Cells

      • Vladimir Granovski, Mario Soares Abreu-Neto, Dimas Tadeu Covas
      Pages 195-202

    3. Purification Method for Recombinant hG-CSF by Affinity Chromatography

      • Bruna Samham Archangelo, Elisa Maria de Sousa Russo
      Pages 203-209

    4. Microplate-Based Method for High-Throughput Screening (HTS) of Chromatographic Conditions Studies for Recombinant Protein Purification

      • Rimenys J. Carvalho, Thayana A. Cruz
      Pages 211-220
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Keywords

About this book

This volume covers a wide spectrum of techniques and approaches that are used in the upstream and downstream processing for recombinant glycoprotein production. Chapters guide the reader through state-of-art of therapeutic recombinant glycoproteins, explores the patent literature, expression systems used for glycoproteins production, methods employed in the downstream processing of different glycoproteins, and information about analytical tools and formulation strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Recombinant Glycoprotein Production: Methods and Protocols aims to ensure successful results in the further study of this vital field

Editors and Affiliations

  • Regional Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil

    Virgínia Picanço-Castro

  • School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

    Kamilla Swiech

Bibliographic Information

  • Book Title: Recombinant Glycoprotein Production

  • Book Subtitle: Methods and Protocols

  • Editors: Virgínia Picanço-Castro, Kamilla Swiech

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7312-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7311-8Published: 19 September 2017

  • Softcover ISBN: 978-1-4939-8449-7Published: 18 August 2018

  • eBook ISBN: 978-1-4939-7312-5Published: 18 September 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 285

  • Number of Illustrations: 14 b/w illustrations, 37 illustrations in colour

  • Topics: Protein Science

  • Industry Sectors: Biotechnology, Pharma

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