Innate Antiviral Immunity

1. Front Matter

   Pages i-xi

   PDF

2. The Application of Humanized Mouse Models for the Study of Human Exclusive Viruses

   • Fatemeh Vahedi, Elizabeth C. Giles, Ali A. Ashkar

   Pages 1-56

3. Zebrafish as a Model for the Study of Host-Virus Interactions

   • Peng Fei Zou, Pin Nie

   Pages 57-78

4. Northern Blot Detection of Virus-Derived Small Interfering RNAs in Caenorhabditis elegans Using Nonradioactive Oligo Probes

   • Tianyun Long, Rui Lu

   Pages 79-88

5. Extraction and qPCR-Based Detection of miRNAs from Cultured PBMCs of Bubaline Origin

   • Chandra S. Mukhopadhyay, Ramneek Verma, Jasdeep Singh

   Pages 89-102

6. Visualizing Virus-Derived dsRNA Using Antibody-Independent and -Dependent Methods

   • Sarah J. Poynter, Stephanie J. DeWitte-Orr

   Pages 103-118

7. RNA PAMPs as Molecular Tools for Evaluating RIG-I Function in Innate Immunity

   • Renee C. Ireton, Courtney Wilkins, Michael Gale Jr.

   Pages 119-129

8. Methods to Visualize MAVS Subcellular Localization

   • Christine Vazquez, Dia C. Beachboard, Stacy M. Horner

   Pages 131-142

9. Purification of Cyclic GMP-AMP from Viruses and Measurement of Its Activity in Cell Culture

   • Alice Mayer, Jonathan Maelfait, Anne Bridgeman, Jan Rehwinkel

   Pages 143-152Open Access

10. cGAMP Quantification in Virus-Infected Human Monocyte-Derived Cells by HPLC-Coupled Tandem Mass Spectrometry

    • Jennifer Paijo, Volkhard Kaever, Ulrich Kalinke

    Pages 153-166

11. Methods of Assessing STING Activation and Trafficking

    • Vladislav Pokatayev, Nan Yan

    Pages 167-174

12. Genome-Wide CRISPR/Cas9 Screening for High-Throughput Functional Genomics in Human Cells

    • Shiyou Zhu, Yuexin Zhou, Wensheng Wei

    Pages 175-181

13. High-Throughput Screening for Identification of Novel Innate Immune Activators

    • Bryan J. Gall, Victor R. DeFilippis

    Pages 183-193

14. Chromosome Conformation Capture for Research on Innate Antiviral Immunity

    • Yoon Jung Kim, Tae Hoon Kim

    Pages 195-208

15. Discovery of Variants Underlying Host Susceptibility to Virus Infection Using Whole-Exome Sequencing

    • Gabriel A. Leiva-Torres, Nestor Nebesio, Silvia M. Vidal

    Pages 209-227

16. Isolation, Purification, and Culture of Primary Murine Sensory Neurons

    • Sarah Katzenell, Jorge R. Cabrera, Brian J. North, David A. Leib

    Pages 229-251

17. Isolation of Group 2 Innate Lymphoid Cells from Mouse Lungs

    • Claudia U. Duerr, Jörg H. Fritz

    Pages 253-261

18. Epidemiological Methods

    • Biao Wang, Mark Loeb

    Pages 263-271

19. Erratum to: Purification of Cyclic GMP-AMP from Viruses and Measurement of Its Activity in Cell Culture

    • Alice Mayer, Jonathan Maelfait, Anne Bridgeman, Jan Rehwinkel

    Pages E1-E1

20. Correction to: Isolation, Purification, and Culture of Primary Murine Sensory Neurons

    • Sarah Katzenell, Jorge R. Cabrera, Brian J. North, David A. Leib

    Pages E3-E3

This book explores methods to study the complex and evolving interplay between a virus and its host that range from model systems to the detection of chemical molecules. The collection starts with the application of humanized mice and zebrafish as model organisms to study virus-host interactions and induction of innate immune responses. Subsequent chapters outline diverse methods to detect small interfering RNAs, microRNAs, and virus-derived dsRNA from a variety of cells, tissues, and organisms, as well as to interrogating the cytosolic RNA and DNA sensing pathways, including using RNA PAMPs as molecular tools, purification of cGAMP from virus particles and infected cells, and mechanisms to visualize the subcellular localization and activation of the adaptor proteins MAVS and STING. Cutting-edge methods, including high-throughput and genome-wide CRISPR/Cas9 screens, chromosome conformation capture, and whole-exome sequencing, are described to identify novel mediators, pathways, and variants underlying host susceptibility. Given the importance of studying these pathways and players under physiologic conditions, methods describing the isolation of primary mouse sensory neurons and group 2 innate lymphoid cells are also provided. Finally, this collection comes full circle back to the whole organism level and concludes with epidemiological methods to investigate virus-host interactions and the induction of innate immunity. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Innate Antiviral Immunity: Methods and Protocols spans a diverse array of approaches to study and elucidate the intricacies of this vital area of study.


The chapter 'Morphological Separation ofClustered Nuclei in Histological Images' is published open access under a CC BY 4.0 license at link.springer.com. 

• Virus-host interactions
• Model organisms
• Infected cells
• Novel pathways
• CRISPR

• Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Canada

  Karen Mossman

Policies and ethics