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  • © 2017

Functional Genomics

Methods and Protocols

  • Includes fully updated, cutting-edge techniques for the study of functional genomics
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1654)

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Table of contents (28 protocols)

  1. Protein Analysis

    1. Front Matter

      Pages 243-243
    2. 2D-DIGE in Proteomics

      • Matias Pasquali, Tommaso Serchi, Sebastien Planchon, Jenny Renaut
      Pages 245-254
    3. STAGE-Diging in Proteomics

      • Paolo Soffientini, Angela Bachi
      Pages 255-260
    4. Protein Arrays I: Antibody Arrays

      • Yulin Yuan, Zuan-Tao Lin, Hongting Wang, Xia Hong, Mikala Heon, Tianfu Wu
      Pages 261-269
    5. Protein Arrays II: Antigen Arrays

      • Yulin Yuan, Hongting Wang, Zuan-Tao Lin, Xia Hong, Mikala Heon, Tianfu Wu
      Pages 271-277
    6. Protein Arrays III: Reverse-Phase Protein Arrays

      • Yulin Yuan, Xia Hong, Zuan-Tao Lin, Hongting Wang, Mikala Heon, Tianfu Wu
      Pages 279-289
    7. Isolation of Exosomes for the Purpose of Protein Cargo Analysis with the Use of Mass Spectrometry

      • Monika Pietrowska, Sonja Funk, Marta Gawin, Łukasz Marczak, Agata Abramowicz, Piotr Widłak et al.
      Pages 291-307
  2. From Genotype to Phenotype

    1. Front Matter

      Pages 309-309
    2. Knockdown of Rice microRNA166 by Short Tandem Target Mimic (STTM)

      • Sachin Teotia, Dabing Zhang, Guiliang Tang
      Pages 337-349
    3. RNAi-Mediated Knockdown of Protein Expression

      • Volker Baumann, Cornelia Lorenzer, Michael Thell, Anna-Maria Winkler, Johannes Winkler
      Pages 351-360
    4. Engineered Zinc Finger DNA-Binding Domains: Synthesis, Assessment of DNA-Binding Affinity, and Direct Protein Delivery to Mammalian Cells

      • Mir A. Hossain, Isaac J. Knudson, Shaleen Thakur, Yong Shen, Jared R. Stees, Joeva J. Barrow et al.
      Pages 361-375
  3. Back Matter

    Pages 389-392

About this book

Reflecting developments in genome editing, this third edition volume fully updates a collection of key techniques for the study of functional genomics. The book is broken up into sections on bioinformatics, DNA, RNA, and protein analysis, as well as a closing section entitled “From Genotype to Phenotype.” Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Updated and authoritative, Functional Genomics: Methods and Protocols, Third Edition seeks to aid scientists in establishing or extending technologies and techniques in their laboratories.

Editors and Affiliations

  • Chair of Biochemistry and Molecular Medicine, Division of Functional Genomics, Faculty of Health, School of Medicine, Center for Biochemical Research and Education ZBAF, Witten/Herdecke University, Witten, Germany

    Michael Kaufmann, Claudia Klinger, Andreas Savelsbergh

Bibliographic Information

  • Book Title: Functional Genomics

  • Book Subtitle: Methods and Protocols

  • Editors: Michael Kaufmann, Claudia Klinger, Andreas Savelsbergh

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7231-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7230-2Published: 07 October 2017

  • Softcover ISBN: 978-1-4939-8424-4Published: 14 August 2018

  • eBook ISBN: 978-1-4939-7231-9Published: 07 October 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XV, 392

  • Number of Illustrations: 23 b/w illustrations, 51 illustrations in colour

  • Topics: Human Genetics

  • Industry Sectors: Biotechnology, Pharma

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access