ErbB Receptor Signaling

1. Front Matter

   Pages i-x

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2. Introduction and Review

   1. Front Matter

      Pages 1-1

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   2. ErbB Receptors and Cancer

      • Zhixiang Wang

      Pages 3-35

   3. New Insights from Drosophila into the Regulation of EGFR Signaling

      • Nicholas Harden

      Pages 37-42

   4. C. elegans Vulva Induction: An In Vivo Model to Study Epidermal Growth Factor Receptor Signaling and Trafficking

      • Kimberley Gauthier, Christian E. Rocheleau

      Pages 43-61

   5. Targeting HER2 in Advanced Breast Cancer

      • Xiaofu Zhu, Anil Abraham Joy

      Pages 63-77

3. Classical Methods in the Studies of ErbB Receptor Signaling

   1. Front Matter

      Pages 79-79

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   2. Methods to Investigate EGFR Ubiquitination

      • Alexia Conte, Sara Sigismund

      Pages 81-100

   3. Dimerization Assessment of Epithelial Growth Factor Family of Receptor Tyrosine Kinases by Using Cross-Linking Reagent

      • Hamid Maadi, Babak Nami, Zhixiang Wang

      Pages 101-108

   4. Application of Immunofluorescence Staining to Study ErbB Family of Receptor Tyrosine Kinases

      • Babak Nami, Zhixiang Wang

      Pages 109-116

   5. Activation of Endosome-Associated Inert EGF Receptor Following Internalization

      • Yi Wang, Sukhmani Billing, Zhixiang Wang

      Pages 117-126

   6. Two-Pulse Endosomal Stimulation of Receptor Tyrosine Kinases Induces Cell Proliferation

      • Steven Pennock, Sukhmani Billing, Zhixiang Wang, Yi Wang

      Pages 127-133

   7. Study of EGFR Signaling/Endocytosis by Site-Directed Mutagenesis

      • Qian Wang, Zhixiang Wang

      Pages 135-143

   8. Using Percoll Gradient Fractionation to Study the Endocytic Trafficking of the EGFR

      • Julie A. Gosney, Brian P. Ceresa

      Pages 145-158

   9. Analysis of Epidermal Growth Factor Receptor-Induced Cell Motility by Wound Healing Assay

      • Junfeng Tong, Zhixiang Wang

      Pages 159-163

4. Recently Developed Methods in the Studies of ErbB Receptor Signaling

   1. Front Matter

      Pages 165-165

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   2. Cell Cycle Synchronization of HeLa Cells to Assay EGFR Pathway Activation

      • Ping Wee, Zhixiang Wang

      Pages 167-181

   3. Analysis of Constitutive EGFR Signaling Regulating IRF3 Transcriptional Activity in Cancer Cells

      • Gao Guo, Ke Gong, Amyn A. Habib

      Pages 183-189

   4. Measurement of Epidermal Growth Factor Receptor-Derived Signals Within Plasma Membrane Clathrin Structures

      • Stefanie Lucarelli, Ralph Christian Delos Santos, Costin N. Antonescu

      Pages 191-225

5. Methods for Studying EGFR Signaling in Drosophila

   1. Front Matter

      Pages 227-227

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This volume contains protocols specifically designed for studying cell signaling mediated by ErbB receptors, all applicable to the study of a broad range of ErbB receptor-mediated signaling from basic research to clinic applications, from cultured cells to various animal models and primary cancer cells from patients. After some overviews, the book continues by covering common protocols for studying various aspects of ErbB receptor-mediated cell signaling, newly-developed methods in biomedical research that are also widely-used in the study of ErbB receptor signaling, epidermal growth factor receptor (EGFR) signaling in Drosophila, as well as protocols for studying ErbB receptor signaling in various animal model systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Detailed and easy-to-follow, ErbB Receptor Signaling: Methods and Protocols serves as an ideal guide for researchers who are studying the cell signaling mediated by ErbB receptors and other receptor tyrosine kinases.

• Cell signaling
• Epidermal growth factor receptor
• Receptor tyrosine kinases
• EGF
• RTKs
• Cancer research
• Animal model systems

• Department of Medical Genetics, University of Alberta, Edmonton, Canada

  Zhixiang Wang

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