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RNA Detection

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Imre Gaspar

    1. Developmental Biology Unit, EMBL Heidelberg, Heidelberg, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1649)

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Table of contents (32 protocols)

  1. Method for Imaging Live-Cell RNA Using an RNA Aptamer and a Fluorescent Probe

    • Shin-ichi Sato, Kenji Yatsuzuka, Yousuke Katsuda, Motonari Uesugi
    Pages 305-318

  2. RNA Live Imaging in the Model Microorganism Ustilago maydis

    • Sabrina Zander, Kira Müntjes, Michael Feldbrügge
    Pages 319-335

  3. Real-Time Fluorescence Imaging of Single-Molecule Endogenous Noncoding RNA in Living Cells

    • Hideaki Yoshimura, Takeaki Ozawa
    Pages 337-347

  4. Live Imaging of mRNA Synthesis in Drosophila

    • Hernan G. Garcia, Thomas Gregor
    Pages 349-357

  5. Imaging Newly Transcribed RNA in Cells by Using a Clickable Azide-Modified UTP Analog

    • Anupam A. Sawant, Sanjeev Galande, Seergazhi G. Srivatsan
    Pages 359-371

  6. Detection of the First Round of Translation: The TRICK Assay

    • Franka Voigt, Jan Eglinger, Jeffrey A. Chao
    Pages 373-384

  7. Imaging Translation Dynamics of Single mRNA Molecules in Live Cells

    • Suzan Ruijtenberg, Tim A. Hoek, Xiaowei Yan, Marvin E. Tanenbaum
    Pages 385-404

  8. Systematic Detection of Poly(A)+ RNA-Interacting Proteins and Their Differential Binding

    • Miha Milek, Markus Landthaler
    Pages 405-417

  9. Isolation and Characterization of Endogenous RNPs from Brain Tissues

    • Rico Schieweck, Foong yee Ang, Renate Fritzsche, Michael A. Kiebler
    Pages 419-426

  10. Individual Nucleotide Resolution UV Cross-Linking and Immunoprecipitation (iCLIP) to Determine Protein–RNA Interactions

    • Christopher R. Sibley
    Pages 427-454

  11. RNA Tagging: Preparation of High-Throughput Sequencing Libraries

    • Christopher P. Lapointe, Marvin Wickens
    Pages 455-471

  12. RAP-MS: A Method to Identify Proteins that Interact Directly with a Specific RNA Molecule in Cells

    • Colleen A. McHugh, Mitchell Guttman
    Pages 473-488

  13. Erratum to: Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction

    • Joshua S. Titlow, Lu Yang, Richard M. Parton, Ana Palanca, Ilan Davis
    Pages E1-E1

  14. Back Matter

    Pages 489-492
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Keywords

About this book

This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs.

Editors and Affiliations

  • Developmental Biology Unit, EMBL Heidelberg, Heidelberg, Germany

    Imre Gaspar

Bibliographic Information

  • Book Title: RNA Detection

  • Book Subtitle: Methods and Protocols

  • Editors: Imre Gaspar

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7213-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7212-8Published: 15 November 2017

  • Softcover ISBN: 978-1-4939-8418-3Published: 30 August 2018

  • eBook ISBN: 978-1-4939-7213-5Published: 11 November 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 492

  • Number of Illustrations: 16 b/w illustrations, 78 illustrations in colour

  • Topics: Human Genetics

  • Industry Sectors: Biotechnology, Pharma

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