Proteomics for Drug Discovery

Methods and Protocols

  • Iulia M. Lazar
  • Maria Kontoyianni
  • Alexandru C. Lazar

Part of the Methods in Molecular Biology book series (MIMB, volume 1647)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Jason R. Thomas, Scott M. Brittain, Jennifer Lipps, Luis Llamas, Rishi K. Jain, Markus Schirle
    Pages 1-18
  3. Yi Chen, Kate J. Fisher, Mark Lloyd, Elizabeth R. Wood, Domenico Coppola, Erin Siegel et al.
    Pages 19-45
  4. Mathias Kalxdorf, Hans Christian Eberl, Marcus Bantscheff
    Pages 47-59
  5. Jacqui L. Mendez-Johnson, Danette L. Daniels, Marjeta Urh, Rachel Friedman Ohana
    Pages 91-108
  6. Kenneth G. Macleod, Bryan Serrels, Neil O. Carragher
    Pages 153-169
  7. Leslie E. W. LaConte, Sarika Srivastava, Konark Mukherjee
    Pages 171-183
  8. A. Cameron Varano, Naoe Harafuji, William Dearnaley, Lisa Guay-Woodford, Deborah F. Kelly
    Pages 185-196
  9. Tuo-Xian Tang, Wen Xiong, Carla V. Finkielstein, Daniel G. S. Capelluto
    Pages 197-206
  10. José Á. Rodríguez-Corrales, Jatinder S. Josan
    Pages 207-219
  11. Alexander Goncearenco, Minghui Li, Franco L. Simonetti, Benjamin A. Shoemaker, Anna R. Panchenko
    Pages 221-236
  12. Shailesh Kumar, Hui Li
    Pages 245-254
  13. Maria Kontoyianni
    Pages 255-266

About this book

Introduction

This book reflects the drug development community’s interest in proteomics methods by providing researchers with the necessary knowledge for the implementation of a number of basic experimental and bioinformatics protocols in the drug discovery research laboratory. The focus of the collection is on select protocols and methodologies that address the analysis of post-translational modifications, targeted protein quantification, protein-protein, protein-lipid, or protein-ATP interactions, in silico lead identification, protein arrays, tissue and cell extract preparation, labeling, chemoproteomics, and drug efficacy assessment. Emphasis was placed not just on methods that describe large-scale protein analysis, but also on ones that are amenable to future implementation in a high-throughput format. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Practical and authoritative, Proteomics for Drug Discovery: Methods and Protocols aims to provide a deep knowledge of the fundamentals of disease biology as well the means that were developed for probing the complex biological milieu.

Keywords

Proteomic platforms Virtual screening Mass spectrometry data Large-scale protein analysis Bioinformatics Drug target discovery Disease biology

Editors and affiliations

  • Iulia M. Lazar
    • 1
  • Maria Kontoyianni
    • 2
  • Alexandru C. Lazar
    • 3
  1. 1.Department of Biological SciencesVirginia TechBlacksburgUSA
  2. 2.Department of Pharmaceutical Sciences, School of PharmacySouthern Illinois University EdwardsvilleEdwardsvilleUSA
  3. 3.ImmunoGenWalthamUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7201-2
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7200-5
  • Online ISBN 978-1-4939-7201-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
Industry Sectors
Chemical Manufacturing
Biotechnology
Consumer Packaged Goods
Pharma