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  • © 2017

Microbial Steroids

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1645)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xi
  2. Steroid Bioconversions

    • Marina V. Donova
    Pages 1-13
  3. Genome Sequencing of Steroid-Producing Bacteria with Illumina Technology

    • Victoria Y. Shtratnikova, Mikhail I. Schelkunov, Marina V. Donova
    Pages 29-44
  4. Genome Sequencing of Steroid Producing Bacteria Using Ion Torrent Technology and a Reference Genome

    • Alberto Sola-Landa, Antonio Rodríguez-García, Carlos Barreiro, Rosario Pérez-Redondo
    Pages 45-58
  5. RNA-Seq-Based Comparative Transcriptomics: RNA Preparation and Bioinformatics

    • Antonio Rodríguez-García, Alberto Sola-Landa, Carlos Barreiro
    Pages 59-72
  6. Intra- and Extra-cellular Proteome Analyses of Steroid-Producer Mycobacteria

    • Carlos Barreiro, Alejandro Morales, Inés Vázquez-Iglesias, Alberto Sola-Landa
    Pages 73-92
  7. Selection of Biodegrading Phytosterol Strains

    • María-Angélica Mondaca, Maricel Vidal, Soledad Chamorro, Gladys Vidal
    Pages 143-150
  8. Stigmasterol Removal by an Aerobic Treatment System

    • Soledad Chamorro, Claudia Xavier, Víctor Hernández, José Becerra, Gladys Vidal
    Pages 151-158
  9. Production and Biotransformation of Phytosterol Microdispersions to Produce 4-Androstene-3,17-Dione

    • Rodrigo A. Mancilla, Roberto Pavez-Díaz, Alejandro Amoroso
    Pages 159-165
  10. β-Sitosterol Bioconversion to Androstenedione in Microtiter Plates

    • Marco P. C. Marques, Pedro Fernandes
    Pages 167-176
  11. Bioconversion of Phytosterols into Androstenedione by Mycobacterium

    • Kjell D. Josefsen, Anna Nordborg, Håvard Sletta
    Pages 177-197
  12. Scale-Up of Phytosterols Bioconversion into Androstenedione

    • Sonia Martínez-Cámara, Esther Bahíllo, José-Luis Barredo, Marta Rodríguez-Sáiz
    Pages 199-210
  13. Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatis CECT 8331

    • Julia García-Fernández, Igor Martínez, Lorena Fernández-Cabezón, Carmen Felpeto-Santero, José-Luis García, Beatriz Galán
    Pages 211-225
  14. Laboratory-Scale Hydroxylation of Steroids by P450BM3 Variants

    • Sabrina Hoebenreich, Martin Spinck, Nathalie Nett
    Pages 239-257
  15. Obtaining of 11α-Hydroxyandrost-4-ene-3,17-dione from Natural Sterols

    • Dmitry Dovbnya, Sergey Khomutov, Vyacheslav Kollerov, Marina V. Donova
    Pages 259-269
  16. Steroid 11-Alpha-Hydroxylation by the Fungi Aspergillus nidulans and Aspergillus ochraceus

    • Lidia Ortega-de los Ríos, José M. Luengo, José M. Fernández-Cañón
    Pages 271-287

About this book

This volume provides practical experimental laboratory protocols for a wide range of steroid bioconversions. The chapters in this book cover topics such as bioconversions and chemical synthesis pathways; strain characterization; bioconversion from sterols to androstenedione and androstadienedione; steroid hydroxylations; biocatalysis; and downstream processes to purify steroid intermediates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Microbial Steroids: Methods and Protocols is a valuable resource for laboratory and industrial professionals. It is also useful for graduate students studying biotechnology, microbiology, genetics, and molecular biology.

Editors and Affiliations

  • Department of Biotechnology, Crystal Pharma, A Division of Albany Molecular Research Inc. (AMRI), Parque Tecnológico de León, León, Spain

    José-Luis Barredo

  • Research and Development Department, Gadea Pharmaceutical Group, A Division of Albany Molecular Research Inc. (AMRI), Parque Tecnológico de Boecillo, Boecillo, Spain

    Ignacio Herráiz

Bibliographic Information

  • Book Title: Microbial Steroids

  • Book Subtitle: Methods and Protocols

  • Editors: José-Luis Barredo, Ignacio Herráiz

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7183-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7182-4Published: 15 July 2017

  • Softcover ISBN: 978-1-4939-8410-7Published: 08 August 2018

  • eBook ISBN: 978-1-4939-7183-1Published: 14 July 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 373

  • Number of Illustrations: 20 b/w illustrations, 27 illustrations in colour

  • Topics: Biochemistry, general

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access