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Current Proteomic Approaches Applied to Brain Function

  • Book
  • © 2017

Overview

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 127)

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About this book

This volume focuses on protein analysis covering a wide spectrum of the utility of mass spectrometry within neurobiological disciplines. The chapters in this book discuss label (iTRAQ, TMT, protein arrays) and label-free workflows (SWATH, MALDI Imaging, and label-free quantitation). Other chapters look at experimental strategies targeted to the identification and quantitation of specific lipids and post-translational modifications, as well as proteomic workflows that focus on characterization of subcellular proteomes. The last few chapters in the book describe various bioinformatics pipelines used to analyze the molecular data derived from high-throughput transcriptomic and proteomic experiments on brain tissue. The Neuromethods series offers chapters with key advice and procedure specifics to empower the readers to successfully achieve their own scientific and experimental goals.

Thorough and comprehensive, Current Proteomic Approaches Applied to Brain Function is a valuable resource for graduate students and postdoctoral fellows interested in neuroproteomics, as well as researchers looking for further insight into the growing field of mass spectrometry in neuroscience.


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Table of contents (21 protocols)

  1. Introduction

  2. Labeling Methods in Neuroproteomics

  3. Label-Free Methods in Neuroproteomics

  4. Mass Spectrometry-Based Neuroproteomics to Analyze Post-Translational Modificiations

  5. Subcellular Neuroproteomics

Editors and Affiliations

  • Clinical Neuroproteomics Unit, Navarrabiomed, Navarra Health Department, Public University of Navarra, Proteored-Institute of Health Carlos III (ISCIII), Navarra Institute for Health Research (IdiSNA), Pamplona, Spain

    Enrique Santamaría, Joaquín Fernández-Irigoyen

Bibliographic Information

  • Book Title: Current Proteomic Approaches Applied to Brain Function

  • Editors: Enrique Santamaría, Joaquín Fernández-Irigoyen

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-7119-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7118-3Published: 27 June 2017

  • Softcover ISBN: 978-1-4939-8390-2Published: 07 August 2018

  • eBook ISBN: 978-1-4939-7119-0Published: 25 June 2017

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XVI, 360

  • Number of Illustrations: 30 b/w illustrations, 61 illustrations in colour

  • Topics: Neurosciences

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Health & Hospitals, Pharma

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