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Zymography

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Jeff Wilkesman

    1. Centre for Environmental, Biology and Chemistry Research, Facultad de Ciencias y Tecnología, University of Carabobo, Valencia, Venezuela

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  2. Liliana Kurz

    1. Centre for Environmental, Biology and Chemistry Research Facultad de Ciencias y Tecnología, University of Carabobo, Valencia, Venezuela

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques for the study of zymography
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1626)

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Table of contents (29 protocols)

  1. Special Zymography Cases

    1. Simultaneous Detection of Activity and Relative Molecular Mass of Adenylate Kinases After SDS-PAGE and Blotting

      • Silvia Ravera, Isabella Panfoli
      Pages 169-178

    2. Silver-Stained Fibrin Zymography: Separation of Proteases and Activity Detection Using a Single Substrate-Containing Gel

      • Chang-Su Park, Dae-Ook Kang, Nack-Shick Choi
      Pages 179-187

    3. Zymography Methods to Simultaneously Analyze Superoxide Dismutase and Catalase Activities: Novel Application for Yeast Species Identification

      • Esther Gamero-Sandemetrio, Rocío Gómez-Pastor, Emilia Matallana
      Pages 189-198

    4. Detection of Guaiacol Peroxidase on Electrophoretic Gels

      • Diana Castro, Lellys M. Contreras, Liliana Kurz, Jeff Wilkesman
      Pages 199-204

    5. In Situ Demonstration and Characteristic Analysis of the Protease Using Substrate Immersing Zymography

      • HaiLun He, Hao Li, Dan Liu
      Pages 205-212

    6. Use of Zymography in Trypanosomiasis Studies

      • Jéssyka Fernanda Santiago Monte, Cláudia Jassica Gonçalves Moreno, Joana Patrícia Molato Figueiredo Lopes Monteiro, Hugo Alexandre de Oliveira Rocha, Aline Rimoldi Ribeiro, Marcelo Sousa Silva
      Pages 213-220

    7. Zymography in Multiwells for Quality Assessment of Proteinases

      • Ambili Mechoor, Madathiparambil G. Madanan
      Pages 221-228

    8. Visualization of Enzyme Activities in Earthworm Biopores by In Situ Soil Zymography

      • Bahar S. Razavi, Duyen Hoang, Yakov Kuzyakov
      Pages 229-238

    9. Multiplex Cathepsin Zymography to Detect Amounts of Active Cathepsins K, L, S, and V

      • Manu O. Platt
      Pages 239-252

    10. Transfer Zymography

      • Daniel Pan, Karl A. Wilson, Anna Tan-Wilson
      Pages 253-269

    11. Sequential Detection of Thermophilic Lipase and Protease by Zymography

      • Liliana Kurz, Zully Hernández, Lellys M. Contreras, Jeff Wilkesman
      Pages 271-277

    12. Calpain Zymography: General Methodology and Protocol

      • Kevin K. W. Wang
      Pages 279-285

    13. CTAB Zymography for the Analysis of Aspartic Proteases from Marine Sponges

      • Oscar González, Jeff Wilkesman
      Pages 287-293

    14. Zymography Detection of a Bacterial Extracellular Thermoalkaline Esterase/Lipase Activity

      • María Tapizquent, Marleny Fernández, Georgina Barreto, Zully Hernández, Lellys M. Contreras, Liliana Kurz et al.
      Pages 295-300

    15. Amylase Zymography

      • Adarelys Andrades, Lellys M. Contreras
      Pages 301-308

  2. Back Matter

    Pages 309-310
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Keywords

About this book

This detailed volume examines the latest techniques and protocols associated with zymography, the practice of detecting enzyme activity on electrophoretic gels. Sections cover subjects such as endopeptidase zymography, reverse zymography & in situ zymography, 2D zymography, as well as a variety of special cases. Written in the highly respected Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and practical, Zymography: Methods and Protocols serves as an ideal aid for a broad array of researchers from the fields of biological and medicinal science, as well as scientists working in clinical and diagnostic medicine, medical genetics, agricultural entomology, genetic monitoring of environmental pollution, and forensic science.

Editors and Affiliations

  • Centre for Environmental, Biology and Chemistry Research, Facultad de Ciencias y Tecnología, University of Carabobo, Valencia, Venezuela

    Jeff Wilkesman

  • Centre for Environmental, Biology and Chemistry Research Facultad de Ciencias y Tecnología, University of Carabobo, Valencia, Venezuela

    Liliana Kurz

Bibliographic Information

  • Book Title: Zymography

  • Book Subtitle: Methods and Protocols

  • Editors: Jeff Wilkesman, Liliana Kurz

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7111-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7109-1Published: 12 June 2017

  • Softcover ISBN: 978-1-4939-8387-2Published: 12 June 2018

  • eBook ISBN: 978-1-4939-7111-4Published: 12 June 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XX, 310

  • Number of Illustrations: 22 b/w illustrations, 45 illustrations in colour

  • Topics: Biochemistry, general

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

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